Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MGSSHHHHHHSSGLVPRGSHMPLLTQQIQDEDDQYSLVASLDNVRNLSTI LKAIHFREHATCFATKNGIKVTVENAKCVQANAFIQAGIFQEFKVQEESV TFRINLTVLLDCLSIFGSSPMPGTLTALRMCYQGYGYPLMLFLEEGGVVT VCKINTQEPEETLDFDFCSTNVINKIILQSEGLREAFSELDMTSEVLQIT MSPDKPYFRLSTFGNAGSSHLDYPKDSDLMEAFHCNQTQVNRYKISLLKP STKALVLSCKVSIRTDNRGFLSLQYMIRNEDGQICFVEYYCCPDEEVPES ES
    • Molecular weight
      34 kDa including tags
    • Amino acids
      1 to 282
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab97946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Mass Spectrometry

    SDS-PAGE

  • Purity
    > 90 % SDS-PAGE.
    ab97946 is purified using conventional chromatography techniques.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 0.2M Sodium chloride, 20mM Tris HCl, 1mM DTT, pH 8.0

General Info

  • Alternative names
    • Cell cycle checkpoint protein Hrad1
    • Cell cycle checkpoint protein Rad 1 A / B
    • Cell cycle checkpoint protein RAD1
    • Checkpoint control protein HRAD1
    • Checkpoint control protein RAD1
    • DNA repair exonuclease
    • DNA repair exonuclease rad1
    • DNA repair exonuclease rad1 homolog
    • DNA repair exonuclease REC1
    • DNA repair protein RAD1
    • EC 3.1.11.2
    • Exonuclease homolog RAD1
    • GTP-binding protein RAD
    • hRAD 1
    • hRAD1
    • MGC77779
    • RAD
    • RAD 1
    • RAD1
    • RAD1 homolog
    • RAD1 homolog (S. pombe)
    • Rad1 like DNA damage checkpoint
    • Rad1 like DNA damage checkpoint protein
    • RAD1, S. pombe, homolog of
    • Rad1-like DNA damage checkpoint protein
    • RAD1_HUMAN
    • Ras associated with diabetes
    • REC 1
    • REC1
    • RRAD
    see all
  • Function
    Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity.
  • Tissue specificity
    Expressed in testis, uterus, bladder, spleen, ovaries, lung, brain and muscle (at protein level).
  • Sequence similarities
    Belongs to the rad1 family.
  • Cellular localization
    Nucleus.
  • Information by UniProt

Images

  • 15% SDS-PAGE showing ab97946 at approximately 33.9kDa (3µg).

References

ab97946 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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