Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionP49459
    • SpeciesHuman
    • SequenceMGSSHHHHHHSSGLVPRGSHMSTPARRRLMRDFKRLQEDPPAGVSGAPSE NNIMVWNAVIFGPEGTPFEDGTFKLTIEFTEEYPNKPPTVRFVSKMFHPN VYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLY QENKREYEKRVSAIVEQSWRDC
    • Molecular weight19 kDa
    • Amino acids1 to 152
    • TagsHis tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab117186 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Immunohistochemistry methylmethacrylate sections

  • Purity> 95 % SDS-PAGE.
    ab117186 is purified by standard chromatography.
  • FormLiquid
  • Additional notesAlthough stable at 4°C for 1 week, ab117186 should be stored desiccated below -18°C. Please prevent freeze thaw cycles.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Please see notes section.

    pH: 8.00
    Constituents: 0.32% Tris HCl, 10% Glycerol, 0.02% DTT, 0.03% EDTA

General Info

  • Alternative names
    • BHR6A
    • hHR6A
    • HR6A
    • mHR6A
    • MRXS30
    • MRXSN
    • RAD6 homolog A
    • RAD6A
    • UBC2
    • UBCD6
    • UBE2A
    • Ube2a ubiquitin-conjugating enzyme E2A
    • UBE2A_HUMAN
    • Ubiquitin carrier protein
    • Ubiquitin carrier protein A
    • Ubiquitin conjugating enzyme E2 17 kDa
    • Ubiquitin conjugating enzyme E2 21.5 kDa
    • Ubiquitin conjugating enzyme E2A
    • Ubiquitin conjugating enzyme E2A (RAD6 homolog)
    • Ubiquitin protein ligase A
    • Ubiquitin-conjugating enzyme E2 A
    • Ubiquitin-protein ligase A
    see all
  • FunctionAccepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
  • Information by UniProt

References for Recombinant Human Rad6 protein (ab117186)

ab117186 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"