Recombinant Human Ran (mutated E70 A) protein (ab90203)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • Modificationsmutated E70A

Specifications

Our Abpromise guarantee covers the use of ab90203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityProtein preparation is 85% GDP-loaded, measured by HPLC.
  • Applications

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.

  • FormLiquid
  • Additional notes


    The mutation E70A results in inhibition of the guanine exchange reaction mediated by RCC1, the guanine nucleotide exchange factor (GEF) for Ran.

    Protein preparation is 85% GDP-loaded, measured by HPLC.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 5mM Magnesium chloride, 20mM Potassium phosphate, 1mM DTE (1, 4-Dithioerythritol), pH 7.4

General Info

  • Alternative names
    • Androgen receptor associated protein 24
    • Androgen receptor-associated protein 24
    • ARA 24
    • ARA24
    • Gsp1
    • GTP binding nuclear protein RAN
    • GTP-binding nuclear protein Ran
    • GTPase Ran
    • Guanosine triphosphatase Ran
    • LPS
    • OK/SW-cl.81
    • ran
    • RAN member RAS oncogene family
    • RAN_HUMAN
    • RanGTPase
    • Ras like protein TC4
    • Ras related nuclear protein
    • Ras-like protein TC4
    • Ras-related nuclear protein
    • RASL2 8
    • TC 4
    • TC4
    see all
  • FunctionGTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules.
    Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases.
  • Tissue specificityExpressed in a variety of tissues.
  • Sequence similaritiesBelongs to the small GTPase superfamily. Ran family.
  • Post-translational
    modifications
    The N-terminus is blocked.
  • Cellular localizationNucleus. Cytoplasm. Melanosome. Becomes dispersed throughout the cytoplasm during mitosis. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

References for Recombinant Human Ran (mutated E70 A) protein (ab90203)

ab90203 has not yet been referenced specifically in any publications.

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