Recombinant Human RAP80 protein (Biotin) (ab189682)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      9 kDa
    • Additional sequence information
      UIM Domains (NP_057374).
  • Conjugation
    Biotin

Specifications

Our Abpromise guarantee covers the use of ab189682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Purity
    >95% by SDS-PAGE .

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 1.19% HEPES, 1.45% Sodium chloride, 0.02% DTT, 5% Glycerol

General Info

  • Alternative names
    • BRCA1-A complex subunit RAP80
    • Nuclear zinc finger protein RAP80
    • OTTHUMP00000161441
    • OTTHUMP00000223372
    • OTTHUMP00000223374
    • RAP80
    • Receptor associated protein 80
    • Receptor-associated protein 80
    • Retinoid x receptor interacting protein
    • Retinoid X receptor interacting protein 110
    • Retinoid X receptor-interacting protein 110
    • RIP110
    • Rxrip110
    • Ubiquitin interaction motif containing 1
    • Ubiquitin interaction motif containing protein 1
    • Ubiquitin interaction motif-containing protein 1
    • UIMC1
    • UIMC1_HUMAN
    • X2HRIP110
    see all
  • Function
    Ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly Act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1.
  • Tissue specificity
    Expressed in testis, ovary, thymus and heart. Expressed in germ cells of the testis.
  • Sequence similarities
    Belongs to the RAP80 family.
    Contains 2 UIM (ubiquitin-interacting motif) repeats.
  • Domain
    The UIM-linker region between the 2 UIM repeats determines the selectivity for 'Lys-63'-linked ubiquitin. The length of the linker is important. The linker reduces the flexibility between the UIM repeats and promotes high-affinity and linkage-selective interactions.
    The Abraxas-interacting region (AIR) mediates the interaction with FAM175A/Abraxas.
  • Post-translational
    modifications
    Sumoylated.
    Phosphorylated upon DNA damage by ATM or ATR.
  • Cellular localization
    Nucleus. Localizes at sites of DNA damage at double-strand breaks.
  • Information by UniProt

References

ab189682 has not yet been referenced specifically in any publications.

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