Overview

  • Product nameRecombinant Human Recoverin protein
  • Protein lengthFull length protein

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceMGNSKSGALS KEILEELQLN TKFSEEELCS WYQSFLKDCP TGRITQQQFQ SIYAKFFPDT DPKAYAQHVF RSFDSNLDGT LDFKEYVIAL HMTTAGKTNQ KLEWAFSLYD VDGNGTISKN EVLEIVMAIF KMITPEDVKL LPDDENTPEK RAEKIWKYFG KNDDDKLTEK EFIEGTLANK EILRLIQFEP QKVKEKMKNA
    • Amino acids1 to 200

Specifications

Our Abpromise guarantee covers the use of ab48757 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.
    Recombinant human recoverin was overexpressed in E. coli and purified by using conventional chromatography techniques.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris, 2mM Magnesium chloride, 1mM EDTA, pH 8.0

General Info

  • Alternative names
    • 23 kDa photoreceptor cell-specific protein
    • Cancer associated retinopathy protein
    • Cancer-associated retinopathy protein
    • CAR
    • CAR protein
    • p26
    • Protein CAR
    • RCV1
    • RCVRN
    • RECO_HUMAN
    • Recoverin
    • S-modulin
    see all
  • FunctionSeems to be implicated in the pathway from retinal rod guanylate cyclase to rhodopsin. May be involved in the inhibition of the phosphorylation of rhodopsin in a calcium-dependent manner. The calcium-bound recoverin prolongs the photoresponse.
  • Tissue specificityRetina and pineal gland.
  • Sequence similaritiesBelongs to the recoverin family.
    Contains 4 EF-hand domains.
  • Information by UniProt

Recombinant Human Recoverin protein images

  • 14% SDS-PAGE gel loaded with recombinant human Recoverin protein.Predicted molecular weight 23.1 kDa.

References for Recombinant Human Recoverin protein (ab48757)

This product has been referenced in:
  • Nakajima E  et al. Calpain, not caspase, is the causative protease for hypoxic damage in cultured monkey retinal cells. Invest Ophthalmol Vis Sci 52:7059-67 (2011). Read more (PubMed: 21757584) »

See 1 Publication for this product

Product Wall

Abreviews
Application Western blot

Review text: Recombinant recoverin used as a positive control for anti-recoverin assay
Sample: Human Recombinant protein (serum)
Loading amount: 1 µg
Specification: serum
Gel Running Conditions: Reduced Denaturing
Blocking step: Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details
Incubation time: 2 hour(s) and 0 minute(s) · Temperature: 25°C

Primary antibody (in addition to 'Recoverin protein')
Primary antibody: Abcam primary antibody: Anti-Recoverin antibody (ab85292)
Dilution: 1/250

Secondary antibody
Name: Non-Abcam antibody was used: anti-rabbit Igs
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution: 1/500

Detection
Detection method: chloronaphthol
Exposure: 20 minute(s) and 0 second(s)
Bands: Specific: 23 kDa
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Submitted Jul 06 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"