Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceMRGSHHHHHH GMASHMEEPQ RELPSARIRC PKGSKAYGSH CYALFLSPKS WTDADLACQK RPSGNLVSVL SGAEGSFVSS LVKSIGNSYS YVWIGLHDPT QGTEPNGEGW EWSSSDVMNY FAWERNPSTI SSPGHCASLS RSTAFLRWKD YNCNVRLPYV CKFTD
    • Molecular weight18 kDa
    • Amino acids27 to 175
    • Additional sequence informationThe Recombinant Human REG3A protein is produced with N-terminal fusion of His Tag. The REG3A His -Tagged Fusion Protein is an 18.4 kDa protein containing 149 amino acid residues

Specifications

Our Abpromise guarantee covers the use of ab73486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

  • Purity> 95 % SDS-PAGE.
    ab73486 is purified by a one-step procedure using affinity Ni-NTA chromatography.
  • FormLyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 0.05M Acetate buffer, pH4

  • ReconstitutionAdd 0.2 ml of 0.1M Acetate buffer pH-4 and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10µg/ml. In higher concentrations the solubility of this antigen is limited.

General Info

  • Alternative names
    • FLJ18565
    • Hepatocarcinoma intestine pancreas
    • Hepatointestinal pancreatic protein
    • HIP
    • HIP/PAP
    • Human proislet peptide
    • INGAP
    • Pancreatic beta cell growth factor
    • Pancreatitis associated protein
    • Pancreatitis associated protein 1
    • Pancreatitis-associated protein 1
    • PAP
    • PAP H
    • PAP homologous protein
    • PAP1
    • PBCGF
    • Proliferation inducing protein 34
    • Proliferation inducing protein 42
    • REG III
    • Reg III alpha
    • Reg III-alpha
    • REG-3-alpha
    • REG3
    • Reg3a
    • REG3A_HUMAN
    • Regenerating islet derived 3 alpha
    • Regenerating islet derived protein 3 alpha precursor
    • Regenerating islet-derived protein 3-alpha 15 kDa form
    • Regenerating islet-derived protein III-alpha
    see all
  • FunctionBactericidal C-type lectin which acts exclusively against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan. Regulates keratinocyte proliferation and differentiation after skin injury via activation of EXTL3-PI3K-AKT signaling pathway.
  • Tissue specificityHighly expressed in epidermal keratinocytes of psoriasis patients (at protein level). Constitutively expressed in intestine. Low expression is found in healthy pancreas. Overexpressed during the acute phase of pancreatitis and in some patients with chronic pancreatitis.
  • Sequence similaritiesContains 1 C-type lectin domain.
  • DomainThe EPN motif is essential for recognition of the peptidoglycan carbohydrate backbone and for efficient bacterial killing with Glu-114 playing a key role in peptidoglycan binding and bactericidal activity.
  • Post-translational
    modifications
    Proteolytic processing by trypsin removes an inhibitory N-terminal propeptide and is essential for peptidoglycan binding and antibacterial activity.
  • Cellular localizationSecreted. Found in the apical region of pancreatic acinar cells.
  • Information by UniProt

References for Recombinant Human Reg3a protein (ab73486)

This product has been referenced in:
  • Cui W  et al. Overexpression of Reg3alpha increases cell growth and the levels of cyclin D1 and CDK4 in insulinoma cells. Growth Factors 27:195-202 (2009). WB . Read more (PubMed: 19343564) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"