Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionP61586
    • SpeciesHuman
    • Molecular weight23 kDa including tags
    • Amino acids1 to 193
    • TagsHis tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab101594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Purity> 85 % SDS-PAGE.
    The purity was determined to be >85% by densitometry
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: 150mM Imidazole
    Constituents: 25% Glycerol, 50mM MOPS, 300mM Sodium chloride, 0.25mM DTT, 0.1mM PMSF, pH 7.0

General Info

  • Alternative names
    • Aplysia ras related homolog 12
    • ARH12
    • ARHA
    • H 12
    • H12
    • Oncogene RHO H12
    • Ras homolog family member A
    • Ras homolog gene family member A
    • Rho A
    • Rho cDNA clone 12
    • RHO H12
    • RHO12
    • RHOA
    • RHOA_HUMAN
    • RHOH12
    • Small GTP binding protein Rho A
    • Transforming protein Rho A
    • Transforming protein RhoA
    see all
  • FunctionRegulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP.
  • Sequence similaritiesBelongs to the small GTPase superfamily. Rho family.
  • DomainThe basic-rich region is essential for yopT recognition and cleavage.
  • Post-translational
    modifications
    Substrate for botulinum ADP-ribosyltransferase.
    Cleaved by yopT protease when the cell is infected by some Yersinia pathogens. This removes the lipid attachment, and leads to its displacement from plasma membrane and to subsequent cytoskeleton cleavage.
    AMPylation at Tyr-34 and Thr-37 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
    Ubiquitinated by the BCR(BACURD1) and BCR(BACURD2) E3 ubiquitin ligase complexes, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration.
  • Cellular localizationCell membrane. Cytoplasm > cytoskeleton.
  • Information by UniProt

Recombinant Human RhoA protein images

  • SDS-PAGE analysis of ab101594

References for Recombinant Human RhoA protein (ab101594)

ab101594 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab101594.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"