The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Protein concentration is above or equal to 0.05 mg/ml.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00 Constituents: 0.31% Glutathione, 0.79% Tris HCl
Putative E3 ubiquitin-protein ligase UNKL
RING finger protein unkempt-like
Unkempt homolog (Drosophila) like
Unkempt like protein
Zinc finger CCCH domain-containing protein 5-like
May participate in a protein complex showing an E3 ligase activity regulated by RAC1. Ubiquitination is directed towards itself and possibly other substrates, such as SMARCD2/BAF60b. Intrinsic E3 ligase activity has not been proven.
Protein modification; protein ubiquitination.
Belongs to the unkempt family. Contains 4 C3H1-type zinc fingers. Contains 1 RING-type zinc finger.
Although this protein contains a RING domain, intrinsic E3 ligase activity has not been proven (PubMed:20148946).
Isoform 4 is ubiquitinated in the C-terminal. Ubiquitination is enhanced by activated RAC1. The presence of the RING finger domain is not essential for ubiquitination to occur.
Cytoplasm. Nucleus. Isoform 4 is primarily localized in the cytoplasm but has the ability to shuttle between the nucleus and the cytoplasm.