The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Protein concentration is above or equal to 0.05 mg/ml.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00 Constituents: 0.31% Glutathione, 0.79% Tris HCl
E3 ubiquitin protein ligase RING 2
E3 ubiquitin protein ligase RING2
E3 ubiquitin-protein ligase RING2
HIP2 interacting protein 3
HIP2-interacting protein 3
Huntingtin interacting protein 2 interacting protein 3
Huntingtin-interacting protein 2-interacting protein 3
Polycomb M33 interacting protein Ring 1B
Polycomb M33 interacting protein Ring1B
RING finger protein 1B
RING finger protein 2
RING finger protein BAP 1
RING finger protein BAP-1
RING finger protein BAP1
E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4. Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity.
Protein modification; protein ubiquitination.
Contains 1 RING-type zinc finger.
Polyubiquitinated in the presence of UBE2D3 (in vitro). Monoubiquitinated, by auto-ubiquitination.
Nucleus. Chromosome. Enriched on inactive X chromosome (Xi) in female trophoblast stem (TS) cells as well as differentiating embryonic stem (ES) cells. The enrichment on Xi is transient during TS and ES cell differentiation. The association with Xi is mitotically stable in non-differentiated TS cells.