Recombinant human RIP3 protein (Tagged) (ab125566)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 70% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: WB, Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human RIP3 protein (Tagged)
See all RIP3 proteins and peptides -
Biological activity
The specific activity of ab125566 was determined to be 38nmol/min/mg.
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Purity
> 70 % Densitometry.
Purity was determined to be >70% by densitometry. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
96 kDa including tags -
Amino acids
1 to 518 -
Tags
GST tag N-Terminus
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Associated products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab125566 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Receptor interacting protein 3
- Receptor interacting serine threonine kinase 3
- Receptor interacting serine/threonine protein kinase 3
see all -
Function
Promotes apoptosis. -
Tissue specificity
Detected at lower levels in heart, placenta, lung and kidney. Isoform 3 is significantly increased in colon and lung cancers. -
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. -
Cellular localization
Cytoplasm. - Information by UniProt
Images
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The specific activity of RIP3 (ab125566) was determined to be 10.6 nmol/min/mg as per activity assay protocol and was equivalent to 32 nmol/min/mg as per radiometric assay
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SDS PAGE analysis of ab125566
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SDS PAGE analysis of ab125566
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SDS-PAGE analysis of ab125566.
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The specific activity of ab125566 was determined to be 38 nmol/min/mg.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab125566 has been referenced in 2 publications.
- Lamade AM et al. Inactivation of RIP3 kinase sensitizes to 15LOX/PEBP1-mediated ferroptotic death. Redox Biol 50:102232 (2022). PubMed: 35101798
- Choi SW et al. PELI1 Selectively Targets Kinase-Active RIP3 for Ubiquitylation-Dependent Proteasomal Degradation. Mol Cell 70:920-935.e7 (2018). PubMed: 29883609