Recombinant Human RNA Polymerase II p14.5 protein (ab81852)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman

Specifications

Our Abpromise guarantee covers the use of ab81852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 20% Glycerol, 20mM Tris Cl, 100mM Potassium chloride, 1mM DTT, 0.2mM EDTA, pH 8.0

General Info

  • Alternative names
    • DNA directed RNA polymerase II 14.5 kDa polypeptide
    • DNA directed RNA polymerase II polypeptide I
    • DNA directed RNA polymerase II subunit I
    • DNA directed RNA polymerase II subunit RPB9
    • DNA-directed RNA polymerase II subunit I
    • DNA-directed RNA polymerase II subunit RPB9
    • hRPB14.5
    • POLR2I
    • Polymerase (RNA) II (DNA directed) polypeptide I 14.5kDa
    • RNA polymerase II 14.5 kDa subunit
    • RNA polymerase II subunit B9
    • RPB14.5
    • RPB9
    • RPB9_HUMAN
    see all
  • FunctionDNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB9 is part of the upper jaw surrounding the central large cleft and thought to grab the incoming DNA template.
  • Sequence similaritiesBelongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.
    Contains 1 TFIIS-type zinc finger.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt

References for Recombinant Human RNA Polymerase II p14.5 protein (ab81852)

ab81852 has not yet been referenced specifically in any publications.

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