Recombinant Human RNF146 protein (ab153534)
Key features and details
- Expression system: Wheat germ
- Tags: GST tag N-Terminus
- Suitable for: ELISA, WB
Description
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Product name
Recombinant Human RNF146 protein -
Expression system
Wheat germ -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MAGCGEIDHSINMLPTNRKANESCSNTAPSLTVPECAICLQTCVHPVSLP CKHVFCYLCVKGASWLGKRCALCRQEIPEDFLDKPTLLSPEELKAASRGN GEYAWYYEGRNGWWQYDERTSRELEDAFSKGKKNTEMLIAGFLYVADLEN MVQYRRNEHGRRRKIKRDIIDIPKKGVAGLRLDCDANTVNLARESSADGA DSVSAQSGASVQPLVSSVRPLTSVDGQLTSPATPSPDASTSLEDSFAHLQ LSGDNTAERSHRGEGEEDHESPSSGRVPAPDTSIEETESDASSDSEDVSA VVAQHSLTQQRLLVSNANQTVPDRSDRSGTDRSVAGGGTVSVSVRSRRPD GQCTVTEV -
Amino acids
1 to 358 -
Tags
GST tag N-Terminus
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Specifications
Our Abpromise guarantee covers the use of ab153534 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
ELISA
Western blot
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Form
Liquid -
Additional notes
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.31% Glutathione, 0.79% Tris HCl
General Info
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Alternative names
- Dactylidin
- dJ351K20.1
- DKFZP434O1427
see all -
Function
E3 ubiquitin-protein ligase that specifically binds poly-ADP-ribosylated proteins and mediates their ubiquitination and subsequent degradation. Acts as an activator of the Wnt signaling pathway by mediating the ubiquitination of poly-ADP-ribosylated AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex. Acts in cooperation with tankyrase proteins (TNKS and TNKS2), which mediate poly-ADP-ribosylation of target proteins AXIN1, AXIN2, BLZF1, CASC3, TNKS and TNKS2. Recognizes and binds tankyrase-dependent poly-ADP-ribosylated proteins via its WWE domain and mediates their ubiquitination. -
Tissue specificity
Ubiquitously expressed. Up-regulated in brains from patients with Alzheimer disease. -
Pathway
Protein modification; protein ubiquitination. -
Involvement in disease
Note=Defects in RNF146 are a cause of susceptibility to breast cancer. -
Sequence similarities
Contains 1 RING-type zinc finger.
Contains 1 WWE domain. -
Domain
The WWE domain mediates non-covalent poly(ADP-ribose)-binding. -
Post-translational
modificationsUbiquitinated; autoubiquitinated. Autoubiquitination is enhanced upon poly(ADP-ribose)-binding. -
Cellular localization
Cytoplasm > cytosol. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab153534 has not yet been referenced specifically in any publications.