Overview

  • Product name
    Recombinant Human RNF34 protein
  • Protein length
    Full length protein

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MGSSHHHHHH SSGLVPRGSH MGSMRKAGAT SMWASCCGLL NEVMGTGAVR GQQSAFAGAT GPFRFTPNPE FSTYPPAATE GPNIVCKACG LSFSVFRKKH VCCDCKKDFC SVCSVLQENL RRCSTCHLLQ ETAFQRPQLM RLKVKDLRQY LILRNIPIDT CREKEDLVDL VLCHHGLGSE DDMDTSSLNS SRSQTSSFFT RSFFSNYTAP SATMSSFQGE LMDGDQTSRS GVPAQVQSEI TSANTEDDDD DDDEDDDDEE ENAEDRNPGL SKERVRASLS DLSSLDDVEG MSVRQLKEIL ARNFVNYSGC CEKWELVEKV NRLYKENEEN QKSYGERLQL QDEEDDSLCR ICMDAVIDCV LLECGHMVTC TKCGKRMSEC PICRQYVVRA VHVFKS
    • Molecular weight
      44 kDa including tags
    • Amino acids
      1 to 373
    • Tags
      His tag N-Terminus
    • Additional sequence information
      Isoform 2. NP_919247.1.

Specifications

Our Abpromise guarantee covers the use of ab176063 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF
  • Purity
    >90% by SDS-PAGE.

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Information available upon request.

General Info

  • Alternative names
    • CARP 1
    • CARP-1
    • CARP1
    • Caspase regulator CARP1
    • Caspases-8 and -10-associated RING finger protein 1
    • E3 ubiquitin-protein ligase RNF34
    • FYVE-RING finger protein Momo
    • hRFI
    • Human RING finger homologous to inhibitor of apoptosis protein
    • RFI
    • RIF
    • RIFF
    • RING finger protein 34
    • RING finger protein RIFF
    • RNF34
    • RNF34_HUMAN
    see all
  • Function
    E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate cell death downstream of death domain receptors in the extrinsic pathway of apoptosis (PubMed:15069192). May mediate 'Lys-48'-linked polyubiquitination of RIPK1 and its subsequent proteasomal degradation thereby indirectly regulating the tumor necrosis factor-mediated signaling pathway (Ref.13). Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation (PubMed:17121812). Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN (PubMed:18382127). Mediates PPARGC1A proteasomal degradation probably through ubiquitination thereby indirectly regulating the metabolism of brown fat cells (PubMed:22064484). Possibly involved in innate immunity, through 'Lys-48'-linked polyubiquitination of NOD1 and its subsequent proteasomal degradation (PubMed:25012219).
  • Tissue specificity
    Ubiquitous. Detected in heart, brain, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, colon and leukocytes.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Contains 1 FYVE-type zinc finger.
    Contains 1 RING-type zinc finger.
    Contains 2 SAP domains.
  • Domain
    The RING-type zinc finger is required for the ubiquitination of target proteins.
    The FYVE-type zinc finger domain is required for localization and may confer affinity for cellular compartments enriched in phosphatidylinositol 5-phosphate and phosphatidylinositol 3-phosphate phospholipids.
  • Post-translational
    modifications
    Autoubiquitinated (in vitro).
    Proteolytically cleaved by caspases upon induction of apoptosis by TNF.
  • Cellular localization
    Cell membrane. Endomembrane system. Nucleus. Nucleus speckle. Cytoplasm, cytosol.
  • Information by UniProt

Images

  • 15% SDS-PAGE analysis of ab176063 (3μg).

References

ab176063 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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