Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      66 kDa including tags
    • Amino acids
      5 to 554
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab125828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    The specific activity of ab125828 was determined to be 605 nmol/min/mg.
  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Purity
    > 95 % Densitometry.
    Purity was determined to be >95% by densitometry.
  • Form
    Liquid
  • Additional notes

    ab204880 (RPS6 peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.00
    Preservative: 1.02% Imidazole
    Constituents: 0.002% PMSF, 0.71% Sodium phosphate, 0.004% DTT, 25% Glycerol, 1.75% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • coiled-coil-containing protein kinase 2
    • KIAA0619
    • p164 ROCK 2
    • p164 ROCK-2
    • Rho associated coiled coil containing protein kinase 2
    • Rho associated protein kinase 2
    • Rho associated, coiled coil containing protein kinase II
    • Rho kinase 2
    • Rho-associated
    • Rho-associated protein kinase 2
    • ROCK 2
    • Rock II
    • Rock2
    • ROCK2_HUMAN
    • Rock2m
    • ROK alpha
    • ROKalpha
    see all
  • Function
    Regulates the assembly of the actin cytoskeleton. Promotes formation of stress fibers and of focal adhesion complexes. Plays a role in smooth muscle contraction.
  • Sequence similarities
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
    Contains 1 REM (Hr1) repeat.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm. Cell membrane. Cytoplasmic, and associated with actin microfilaments and the plasma membrane.
  • Information by UniProt

Images

  • SDS-PAGE analysis of ab125828.
  • The specific activity of ab125828 was determined to be 605 nmol/min/mg.

References

ab125828 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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