Recombinant human ROCK2 protein (ab51423)

Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • SpeciesHuman
    • Amino acids5 to 554

Associated products

Specifications

Our Abpromise guarantee covers the use of ab51423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activitySpecific activity 350 nmol/min/mg.
  • Applications

    Functional Studies

    SDS-PAGE

  • FormLiquid
  • Additional notes

    ab204880 (RPS6 peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • coiled-coil-containing protein kinase 2
    • KIAA0619
    • p164 ROCK 2
    • p164 ROCK-2
    • Rho associated coiled coil containing protein kinase 2
    • Rho associated protein kinase 2
    • Rho associated, coiled coil containing protein kinase II
    • Rho kinase 2
    • Rho-associated
    • Rho-associated protein kinase 2
    • ROCK 2
    • Rock II
    • Rock2
    • ROCK2_HUMAN
    • Rock2m
    • ROK alpha
    • ROKalpha
    see all
  • FunctionRegulates the assembly of the actin cytoskeleton. Promotes formation of stress fibers and of focal adhesion complexes. Plays a role in smooth muscle contraction.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
    Contains 1 REM (Hr1) repeat.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm. Cell membrane. Cytoplasmic, and associated with actin microfilaments and the plasma membrane.
  • Information by UniProt

Recombinant human ROCK2 protein images

  • Kinase activity assay - specific activity 350 nmol/min/mg
  • Recombinant human ROCK2 protein (tagged)
    Molecular weight 88 kDa

References for Recombinant human ROCK2 protein (ab51423)

ab51423 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us with your question. The molecular weight of the protein plus tag is 88 kDa. I hope this is helpful, but please let me know if you have further questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"