Recombinant Human RRAS2 protein (ab87714)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      MGSSHHHHHH SSGLVPRGSH MAAAGWRDGS GQEKYRLVVV GGGGVGKSAL TIQFIQSYFV TDYDPTIEDS YTKQCVIDDR AARLDILDTA GQEEFGAMRE QYMRTGEGFL LVFSVTDRGS FEEIYKFQRQ ILRVKDRDEF PMILIGNKAD LDHQRQVTQE EGQQLARQLK VTYMEASAKI RMNVDQAFHE LVRVIRKFQE QECPPSPEPT RKEKDKKGCH C

Specifications

Our Abpromise guarantee covers the use of ab87714 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity
    > 95 % SDS-PAGE.
    ab87714 is purified using conventional chromatography techniques.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, 2mM EDTA, pH 8.0

General Info

  • Alternative names
    • C86394
    • Oncogene RRAS2
    • Oncogene TC21
    • Ras like protein TC21
    • Ras related protein R Ras2
    • Ras-like protein TC21
    • Ras-related protein R-Ras2
    • Related RAS viral (r ras) oncogene homolog 2
    • Related RAS viral oncogene homolog 2
    • RRAS 2
    • RRAS2
    • RRAS2_HUMAN
    • TC 21
    • TC21
    • Teratocarcinoma oncogene
    • Teratocarcinoma oncogene TC21
    see all
  • Function
    It is a plasma membrane-associated GTP-binding protein with GTPase activity. Might transduce growth inhibitory signals across the cell membrane, exerting its effect through an effector shared with the Ras proteins but in an antagonistic fashion.
  • Tissue specificity
    Ubiquitously present in all tissues examined, with the highest levels in heart, placenta, and skeletal muscle. Moderate levels in lung and liver; low levels in brain, kidney, and pancreas.
  • Involvement in disease
    Ovarian cancer
  • Sequence similarities
    Belongs to the small GTPase superfamily. Ras family.
  • Post-translational
    modifications
    May be post-translationally modified by both palmitoylation and polyisoprenylation.
  • Cellular localization
    Cell membrane. Inner surface of plasma membrane possibly with attachment requiring acylation of the C-terminal cysteine (By similarity with RAS).
  • Information by UniProt

Images

  • 15% SDS-PAGE showing ab87714 at approximately 25.2 kDa (3µg).

References

ab87714 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Other
I've used this RRAS2 or TC21 protein for activity assays. TC21 has higher GTPase activity than others members of Ras family. Reading GTP consumption I have recorded good activities (up to 5nM GTP x microg-1 x min-1) without GAP protein. I used also Neurofibromin 1 as GAP and the consumption has increased around 5 times. I also perform coprecipitation assays to pull down activated TC21 with GST-tagged RBD domain of p110delta (in order to measure TC21 activation) and it worked very well with as low as 10ng of TC21. I greatly recommend this product.
Username

Mr. Pedro Campos

Verified customer

Submitted Dec 17 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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