Recombinant human Rsk 2 / MAPKAP Kinase 1b protein (ab60881)

Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Species
      Human

Specifications

Our Abpromise guarantee covers the use of ab60881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Purity
    > 90 % SDS-PAGE.
    Purity: >90% as determined by densitometry.
  • Form
    Liquid
  • Additional notes

    ab204879 (RPS6 peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • 90 kDa ribosomal protein S6 kinase 3
    • CLS
    • HU 3
    • HU2
    • HU3
    • Insulin stimulated protein kinase 1
    • Insulin-stimulated protein kinase 1
    • ISPK-1
    • ISPK1
    • KS6A3_HUMAN
    • MAP kinase activated protein kinase 1b
    • MAP kinase-activated protein kinase 1b
    • MAPK activated protein kinase 1b
    • MAPK-activated protein kinase 1b
    • MAPKAP kinase 1b
    • MAPKAPK 1b
    • MAPKAPK-1b
    • MAPKAPK1B
    • Mental retardation, X linked 19
    • MRX19
    • OTTHUMP00000023036
    • p90 RSK2
    • p90 RSK3
    • p90-RSK 3
    • p90RSK3
    • pp90RSK2
    • Ribosomal protein S6 kinase 90kDa polypeptide 3
    • Ribosomal protein S6 kinase alpha 3
    • Ribosomal protein S6 kinase alpha-3
    • Ribosomal protein s6 kinase ii alpha 2
    • Ribosomal S6 kinase 2
    • Rps6ka3
    • RSK
    • RSK-2
    • RSK2
    • S6 kinase 2
    • S6K alpha3
    • S6K-alpha-3
    see all
  • Function
    Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
  • Tissue specificity
    Expressed in many tissues, highest levels in skeletal muscle.
  • Involvement in disease
    Defects in RPS6KA3 are the cause of Coffin-Lowry syndrome (CLS) [MIM:303600]; an X-linked dominant disorder characterized by severe mental retardation with facial and digital dysmorphisms, and progressive skeletal deformations.
  • Sequence similarities
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 2 protein kinase domains.
  • Post-translational
    modifications
    Autophosphorylated on Ser-386, as part of the activation process.
    Ser-227 phosphorylation promotes Ser-386 phosphorylation and leads to basal activation. Full activation by growth factors requires additional phosphorylation on Ser-369.
  • Information by UniProt

Images

  • Sample Kinase Activity Plot.
  • ab60881 on SDS-PAGE, MW ~112 kDa.

References

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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