Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Species
      Human

Specifications

Our Abpromise guarantee covers the use of ab60882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Purity
    > 90 % SDS-PAGE.
    Purity: >90% as determined by densitometry.
  • Form
    Liquid
  • Additional notes

    ab204879 (RPS6 peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • 90 kDa ribosomal protein S6 kinase 2
    • HU 2
    • KS6A2_HUMAN
    • MAP kinase activated protein kinase 1c
    • MAP kinase-activated protein kinase 1c
    • MAPK-activated protein kinase 1c
    • MAPKAP kinase 1c
    • MAPKAPK-1c
    • MAPKAPK1C
    • Mitogen.activated protein kinase-activated protein kinase 1C
    • p90 RSK3
    • p90-RSK 2
    • p90RSK2
    • pp90RSK3
    • Ribosomal protein S6 kinase alpha-2
    • ribosomal protein S6 kinase, 90kDa, polypeptide 2
    • Ribosomal S6 kinase 3
    • RPS6KA2
    • RSK
    • RSK 3
    • RSK-3
    • S6K alpha
    • S6K alpha 2
    • S6K-alpha-2
    see all
  • Function
    Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of transcription factors, regulates translation, and mediates cellular proliferation, survival, and differentiation. May function as tumor suppressor in epithelial ovarian cancer cells.
  • Tissue specificity
    Widely expressed with higher expression in lung, skeletal muscle, brain, uterus, ovary, thyroid and prostate.
  • Sequence similarities
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 2 protein kinase domains.
  • Post-translational
    modifications
    Activated by phosphorylation at Ser-218 by PDPK1. Autophosphorylated on Ser-377, as part of the activation process. May be phosphorylated at Thr-356 and Ser-360 by MAPK1/ERK2 and MAPK3/ERK1.
    N-terminal myristoylation results in an activated kinase in the absence of added growth factors.
  • Cellular localization
    Nucleus. Cytoplasm.
  • Information by UniProt

Images

  • Sample Kinase Activity Plot.
  • ab60882 on SDS-PAGE, MW ~112 kDa.

References

ab60882 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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