The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Measured by its ability to induce activation of beta-catenin response in a Luciferase assay using HEK293T cells. The ED50 for this effect is typically 0.5 - 2.0 ng/ml in the presence of 5 ng/ml recombinant Wnt3a.
< 1.000 Eu/µg
>95% by SDS-PAGE .
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Protein with TSP type-1 repeat
R-spondin 3 homolog
Roof plate specific spondin-3
Roof plate-specific spondin-3
Thrombospondin type I domain containing 2
Thrombospondin type-1 domain-containing protein 2
Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway, possibly via a direct interaction with Wnt proteins, and in a Wnt-independent beta catenin pathway through a receptor signaling pathway that may not use frizzled/LRP receptors. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway.
Ubiquitously expressed. Expressed at higher level in placenta, small intestine, fetal thymus and lymph node.
Belongs to the R-spondin family. Contains 2 FU (furin-like) repeats. Contains 1 TSP type-1 domain.
The FU repeats are required for activation and stabilization of beta-catenin.