Recombinant Human S adenosylhomocysteine hydrolase protein (ab99326)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionP23526
    • SpeciesHuman
    • SequenceMGSSHHHHHHSSGLVPRGSHMSDKLPYKVADIGLAAWGRKALDIAENEMP GLMRMRERYSASKPLKGARIAGCLHMTVETAVLIETLVTLGAEVQWSSCN IFSTQDHAAAAIAKAGIPVYAWKGETDEEYLWCIEQTLYFKDGPLNMILD DGGDLTNLIHTKYPQLLPGIRGISEETTTGVHNLYKMMANGILKVPAINV NDSVTKSKFDNLYGCRESLIDGIKRATDVMIAGKVAVVAGYGDVGKGCAQ ALRGFGARVIITEIDPINALQAAMEGYEVTTMDEACQEGNIFVTTTGCID IILGRHFEQMKDDAIVCNIGHFDVEIDVKWLNENAVEKVNIKPQVDRYRL KNGRRIILLAEGRLVNLGCAMGHPSFVMSNSFTNQVMAQIELWTHPDKYP VGVHFLPKKLDEAVAEAHLGKLNVKLTKLTEKQAQYLGMSCDGPFKPDHY RY
    • Molecular weight50 kDa including tags
    • Amino acids1 to 432
    • TagsHis tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab99326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF
  • Purity> 95 % SDS-PAGE.
    ab99326 is purified using conventional chromatography techniques.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    pH: 7.40
    Constituents: 20% Glycerol, PBS

General Info

  • Alternative names
    • Adenosylhomocysteinase
    • AdoHcyase
    • ahcY
    • S adenosyl L homocysteine hydrolase
    • S adenosylhomocysteine hydrolase
    • S-adenosyl-L-homocysteine hydrolase
    • SAHH
    • SAHH_HUMAN
    see all
  • FunctionAdenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine.
  • PathwayAmino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1.
  • Involvement in diseaseDefects in AHCY are the cause of hypermethioninemia with S-adenosylhomocysteine hydrolase deficiency (HMAHCHD) [MIM:613752]. A metabolic disorder characterized by hypermethioninemia associated with failure to thrive, mental and motor retardation, facial dysmorphism with abnormal hair and teeth, and myocardiopathy.
  • Sequence similaritiesBelongs to the adenosylhomocysteinase family.
  • Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

Recombinant Human S adenosylhomocysteine hydrolase protein images

  • 15% SDS-PAGE showing ab99326 (3µg).
  • Anti-S adenosylhomocysteine hydrolase antibody - Aminoterminal end (ab56146) at 1 µg/ml + Recombinant Human S adenosylhomocysteine hydrolase protein (ab99326) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 90 seconds

References for Recombinant Human S adenosylhomocysteine hydrolase protein (ab99326)

ab99326 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

please find below the information we managed to collect about the S adenosylhomocysteine hydrolase protein:

Regrettably, our protein’s biological activity hasn’t been tested yet, and we can&rsq...

Read More

Gracias por contactarnos.

Espero que no te importe que te conteste en castellano, ya que recientemente hemos inaugurado el Servicio Técnico en español. Si prefirieses seguir contactándonos en ingles, no dudes en comunic&a...

Read More

Thank you for contacting Abcam.

We have not tested any of these products for enzymatic activity and so we cannot guarantee that they will have. However, you could take advantage of our testing discount program:

http://www.abcam.com/...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"