Recombinant Human S adenosylhomocysteine hydrolase protein (ab99326)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MGSSHHHHHHSSGLVPRGSHMSDKLPYKVADIGLAAWGRKALDIAENEMP GLMRMRERYSASKPLKGARIAGCLHMTVETAVLIETLVTLGAEVQWSSCN IFSTQDHAAAAIAKAGIPVYAWKGETDEEYLWCIEQTLYFKDGPLNMILD DGGDLTNLIHTKYPQLLPGIRGISEETTTGVHNLYKMMANGILKVPAINV NDSVTKSKFDNLYGCRESLIDGIKRATDVMIAGKVAVVAGYGDVGKGCAQ ALRGFGARVIITEIDPINALQAAMEGYEVTTMDEACQEGNIFVTTTGCID IILGRHFEQMKDDAIVCNIGHFDVEIDVKWLNENAVEKVNIKPQVDRYRL KNGRRIILLAEGRLVNLGCAMGHPSFVMSNSFTNQVMAQIELWTHPDKYP VGVHFLPKKLDEAVAEAHLGKLNVKLTKLTEKQAQYLGMSCDGPFKPDHY RY
    • Molecular weight
      50 kDa including tags
    • Amino acids
      1 to 432
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab99326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF
  • Purity
    > 95 % SDS-PAGE.
    ab99326 is purified using conventional chromatography techniques.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    pH: 7.40
    Constituents: 20% Glycerol, PBS

General Info

  • Alternative names
    • Adenosylhomocysteinase
    • AdoHcyase
    • ahcY
    • S adenosyl L homocysteine hydrolase
    • S adenosylhomocysteine hydrolase
    • S-adenosyl-L-homocysteine hydrolase
    • SAHH
    • SAHH_HUMAN
    see all
  • Function
    Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine.
  • Pathway
    Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1.
  • Involvement in disease
    Defects in AHCY are the cause of hypermethioninemia with S-adenosylhomocysteine hydrolase deficiency (HMAHCHD) [MIM:613752]. A metabolic disorder characterized by hypermethioninemia associated with failure to thrive, mental and motor retardation, facial dysmorphism with abnormal hair and teeth, and myocardiopathy.
  • Sequence similarities
    Belongs to the adenosylhomocysteinase family.
  • Cellular localization
    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

Images

  • 15% SDS-PAGE showing ab99326 (3µg).
  • Anti-S adenosylhomocysteine hydrolase antibody - Aminoterminal end (ab56146) at 1 µg/ml + Recombinant Human S adenosylhomocysteine hydrolase protein (ab99326) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 90 seconds

References

This product has been referenced in:
  • Drake KM  et al. A sensitive luminescent assay for the histone methyltransferase NSD1 and other SAM-dependent enzymes. Assay Drug Dev Technol 12:258-71 (2014). Functional Studies . Read more (PubMed: 24927133) »

See 1 Publication for this product

Customer reviews and Q&As

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please find below the information we managed to collect about the S adenosylhomocysteine hydrolase protein:

Regrettably, our protein’s biological activity hasn’t been tested yet, and we can&rsq...

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We have not tested any of these products for enzymatic activity and so we cannot guarantee that they will have. However, you could take advantage of our testing discount program:

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