Recombinant human S100 alpha protein (ab68609)

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Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      MKHHHHHHAS GSELETAMET LINVFHAHSG KEGDKYKLSK KELKELLQTE LSGFLDAQKD VDAVDKVMKE LDENGDGEVD FQEYVVLVAA LTVACNNFFW ENS
    • Molecular weight
      12 kDa including tags
    • Tags
      His tag N-Terminus
    • Additional sequence information
      S100A1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 103 amino acids which include a 10 amino acid His Tag fused at N-terminus.

Specifications

Our Abpromise guarantee covers the use of ab68609 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Activity: ~3 nmol/mg/min using phosphatidylinositol as substrate.
  • Applications

    Western blot

    ELISA

    Inhibition Assay

  • Purity
    > 95 % SDS-PAGE.

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 50mM Sodium chloride, 20mM HEPES-KOH, 1mM DTT, 1mM EDTA, pH 7.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Bpb
    • NEF
    • Protein S100-A1
    • S-100 protein alpha chain
    • S-100 protein subunit alpha
    • S100
    • S100 alpha
    • S100 Alpha Chain
    • S100 Beta Chain
    • S100 Calcium Binding Protein A1
    • S100 Calcium Binding Protein B
    • S100 Calcium Binding Protein Beta Neural
    • S100 calcium-binding protein A1
    • S100 protein alpha polypeptide
    • S100A
    • s100a1
    • S10A1_HUMAN
    see all
  • Function
    Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
  • Tissue specificity
    Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
  • Sequence similarities
    Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt

References for Recombinant human S100 alpha protein (ab68609)

ab68609 has not yet been referenced specifically in any publications.

Publishing research using ab68609? Please let us know so that we can cite the reference in this datasheet.

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