Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionP06702
    • SpeciesHuman
    • SequenceMTCKMSQLER NIETIINTFH QYSVKLGHPD TLNQGEFKEL VRKDLQNFLK KENKNEKVIE HIMEDLDTNA DKQLSFEEFI MLMARLTWAS HEKMHEGDEG PGHHHKPGLG EGTPLEHHHH HH
    • Molecular weight14 kDa including tags
    • Amino acids1 to 114
    • TagsHis tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab95909 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

    Western blot

  • Mass spectrometry
    MALDI-TOF-TOF
  • Purity> 90 % SDS-PAGE.
    ab95909 is purified using conventional chromatography techniques.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, pH 8.0

General Info

  • Alternative names
    • Leukocyte L1 complex heavy chain
    • 60B8AG
    • CAGB
    • Calgranulin B
    • Calgranulin-B
    • Calprotectin L1H subunit
    • CFAG
    • CGLB
    • Cystic fibrosis antigen B
    • L1AG
    • Leukocyte L1 complex heavy chain
    • LIAG
    • MAC387
    • MIF
    • Migration inhibitory factor related protein 14
    • Migration inhibitory factor-related protein 14
    • MRP 14
    • MRP-14
    • MRP14
    • Myeloid-related protein 14
    • NIF
    • OTTHUMP00000015331
    • p14
    • Protein S100-A9
    • S100 A9
    • S100 calcium binding protein A9
    • S100 calcium binding protein A9 calgranulin B
    • S100 calcium-binding protein A9
    • S100A9
    • S10A9_HUMAN
    see all
  • FunctionCalcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization when unphosphorylated. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions.
  • Tissue specificityExpressed by macrophages in acutely inflammed tissues and in chronic inflammation. Detected in peripheral blood leukocytes, in neutrophils and granulocytes. Detected at sites of vascular inflammation (at protein level). Also expressed in epithelial cells constitutively or induced during dermatoses.
  • Sequence similaritiesBelongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Post-translational
    modifications
    Phosphorylated. Phosphorylation inhibits activation of tubulin polymerization.
  • Cellular localizationSecreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation.
  • Information by UniProt

Recombinant Human S100A9 protein images

  • 15% SDS-PAGE showing ab95909 at approximately 14.3kDa (3µg).

References for Recombinant Human S100A9 protein (ab95909)

ab95909 has not yet been referenced specifically in any publications.

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