Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionQ9UBE0
    • SpeciesHuman
    • SequenceMHHHHHHMAS MTGGQQMGRD LYDDDDKDRW GSMVEKEEAG GGISEEEAAQ YDRQIRLWGL EAQKRLRASR VLLVGLKGLG AEIAKNLILA GVKGLTMLDH EQVTPEDPGA QFLIRTGSVG RNRAEASLER AQNLNPMVDV KVDTEDIEKK PESFFTQFDA VCLTCCSRDV IVKVDQICHK NSIKFFTGDV FGYHGYTFAN LGEHEFVEEK TKVAKVSQGV EDGPDTKRAK LDSSETTMVK KKVVFCPVKE ALEVDWSSEK AKAALKRTTS DYFLLQVLLK FRTDKGRDPS SDTYEEDSEL LLQIRNDVLD SLGISPDLLP EDFVRYCFSE MAPVCAVVGG ILAQEIVKAL SQRDPPHNNF FFFDGMKGNG IVECLGPK
    • Molecular weight42 kDa including tags
    • Amino acids1 to 346
    • TagsHis tag N-Terminus , T7 tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab96772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Mass Spectrometry

    SDS-PAGE

  • Mass spectrometry
    MALDI-TOF-TOF
  • Purity> 90 % SDS-PAGE.
    ab96772 is purified using conventional chromatography techniques.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, 1mM DTT, pH 8.0

General Info

  • Alternative names
    • Activator of SUMO1
    • AOS1
    • HSPC140
    • Sae1
    • SAE1_HUMAN
    • Sentrin/SUMO activating protein AOS1
    • SUA1
    • SUMO 1 activating enzyme E1 N subunit
    • SUMO 1 activating enzyme subunit 1
    • SUMO-activating enzyme subunit 1
    • Ubiquitin like protein SUMO1 activating enzyme
    • Ubiquitin-like 1-activating enzyme E1A
    • UBL E1A
    • UBLE1A
    see all
  • FunctionThe heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
  • Tissue specificityExpression level increases during S phase and drops in G2 phase (at protein level).
  • PathwayProtein modification; protein sumoylation.
  • Sequence similaritiesBelongs to the ubiquitin-activating E1 family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt

Recombinant Human SAE1 protein images

  • 15% SDS-PAGE showing ab96772 at approximately 42.2kDa (3µg).

References for Recombinant Human SAE1 protein (ab96772)

ab96772 has not yet been referenced specifically in any publications.

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