Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      71 kDa including tags
    • Amino acids
      1 to 367

Associated products

Specifications

Our Abpromise guarantee covers the use of ab125651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    The specific activity of ab125651 was determined to be 220 nmol/min/mg.
  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Purity
    > 90 % Densitometry.
    Purity was determined to be >90% by densitometry.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • ERK 6
    • ERK-6
    • ERK6
    • Extracellular signal-regulated kinase 6
    • MAP kinase 12
    • MAP kinase p38 gamma
    • MAPK 12
    • Mapk12
    • Mitogen Activated Protein Kinase 12
    • Mitogen activated protein kinase p38 gamma
    • Mitogen-activated protein kinase 12
    • Mitogen-activated protein kinase p38 gamma
    • MK12_HUMAN
    • P38 GAMMA
    • P38GAMMA
    • PRKM12
    • SAPK 3
    • SAPK-3
    • SAPK3
    • Stress Activated Protein Kinase 3
    • Stress-activated protein kinase 3
    see all
  • Function
    Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating downstream targets. Plays a role in myoblast differentiation and also in the down-regulation of cyclin D1 in response to hypoxia in adrenal cells suggesting MAPK12 may inhibit cell proliferation while promoting differentiation.
  • Tissue specificity
    Highly expressed in skeletal muscle and heart.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Domain
    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.
  • Cellular localization
    Cytoplasm. Mitochondrion. Mitochondrial when associated with SH3BP5.
  • Information by UniProt

Images

  • SDS-PAGE analysis of ab125651.
  • The specific activity of ab125651 was determined to be 220 nmol/min/mg by Kinase Assay.

References

ab125651 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab125651.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up