Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      71 kDa including tags
    • Amino acids
      1 to 365

Specifications

Our Abpromise guarantee covers the use of ab125643 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    The specific activity of ab125643 was determined to be 140 nmol/min/mg.
  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Purity
    > 90 % Densitometry.
    Purity was determined to be >90% by densitometry.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • MAP kinase 13
    • MAP kinase p38 delta
    • MAPK 13
    • MAPK-13
    • Mapk13
    • MGC99536
    • Mitogen activated protein kinase 13
    • Mitogen-activated protein kinase 13
    • Mitogen-activated protein kinase p38 delta
    • MK13_HUMAN
    • OTTHUMP00000016282
    • OTTHUMP00000016283
    • p38 delta
    • P38delta
    • PRKM13
    • SAPK 4
    • SAPK4
    • Stress activated protein kinase 4
    • Stress-activated protein kinase 4
    see all
  • Function
    Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating downstream targets. Plays a role in the regulation of protein translation by phosphorylating and inactivating EEF2K.
  • Tissue specificity
    Expressed in testes, pancreas, small intestine, lung and kidney. Abundant in macrophages, also present in neutrophils, CD4+ T-cells, and endothelial cells.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Domain
    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.
  • Information by UniProt

Recombinant human SAPK4 protein images

  • SDS-PAGE analysis of ab125643.
  • The specific activity of ab125643 was determined to be 140 nmol/min/mg by Kinase Assay.

References for Recombinant human SAPK4 protein (ab125643)

ab125643 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab125643.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up