Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      71 kDa including tags
    • Amino acids
      1 to 365

Specifications

Our Abpromise guarantee covers the use of ab125643 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    The specific activity of ab125643 was determined to be 140 nmol/min/mg.
  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Purity
    > 90 % Densitometry.
    Purity was determined to be >90% by densitometry.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • MAP kinase 13
    • MAP kinase p38 delta
    • MAPK 13
    • MAPK-13
    • Mapk13
    • MGC99536
    • Mitogen activated protein kinase 13
    • Mitogen-activated protein kinase 13
    • Mitogen-activated protein kinase p38 delta
    • MK13_HUMAN
    • OTTHUMP00000016282
    • OTTHUMP00000016283
    • p38 delta
    • P38delta
    • PRKM13
    • SAPK 4
    • SAPK4
    • Stress activated protein kinase 4
    • Stress-activated protein kinase 4
    see all
  • Function
    Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating downstream targets. Plays a role in the regulation of protein translation by phosphorylating and inactivating EEF2K.
  • Tissue specificity
    Expressed in testes, pancreas, small intestine, lung and kidney. Abundant in macrophages, also present in neutrophils, CD4+ T-cells, and endothelial cells.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Domain
    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.
  • Information by UniProt

Images

  • SDS-PAGE analysis of ab125643.
  • The specific activity of ab125643 was determined to be 140 nmol/min/mg by Kinase Assay.

References

ab125643 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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