Recombinant Human Secretory phospholipase A2 Type V protein (ab63846)

Overview

  • Product name
    Recombinant Human Secretory phospholipase A2 Type V protein
  • Protein length
    Full length protein

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      MRGSHHHHHH GMASHMGLLD LKSMIEKVTG KNALTNYGFY GCYCGWGGRG TPKDGTDWCC WAHDHCYGRL EEKGCNIRTQ SYKYRFAWGV VTCEPGPFCH VNLCACDRKL VYCLKRNLRS YNPQYQYFPN ILCS
    • Amino acids
      21 to 138

Specifications

Our Abpromise guarantee covers the use of ab63846 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

  • Purity
    > 95 % SDS-PAGE.

  • Form
    Lyophilised
  • Additional notes
    Product is NOT sterile! Please filter the product by an appropriate sterile filter before using it in cell culture.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    0.05M Acetate buffer pH 4.0

  • Reconstitution
    Add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10µg/ml. In higher concentrations the solubility of this antigen is limited.

General Info

  • Alternative names
    • Ca2+ dependent phospholipase A2
    • Calcium dependent phospholipase A2
    • Calcium-dependent phospholipase A2
    • DKFZp686C2294
    • FRFB
    • Group V phospholipase A2
    • GV PLA2
    • gVPLA2
    • hVPLA(2)
    • MGC46205
    • OTTHUMP00000044655
    • PA2G5_HUMAN
    • Phosphatidylcholine 2 acylhydrolase
    • Phosphatidylcholine 2-acylhydrolase 5
    • Phospholipase A2 group V
    • PLA2 10
    • PLA2 G5
    • PLA2-10
    • Pla2g5
    • sPLA2 Type V
    see all
  • Function
    PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. This isozyme hydrolyzes more efficiently L-alpha-1-palmitoyl-2-oleoyl phosphatidylcholine than L-alpha-1-palmitoyl-2-arachidonyl phosphatidylcholine, L-alpha-1-palmitoyl-2-arachidonyl phosphatidylethanolamine, or L-alpha-1-stearoyl-2-arachidonyl phosphatidylinositol. May be involved in the production of lung surfactant, the remodeling or regulation of cardiac muscle.
  • Tissue specificity
    Heart, placenta and less abundantly, in lung.
  • Sequence similarities
    Belongs to the phospholipase A2 family.
  • Post-translational
    modifications
    This enzyme lacks one of the seven disulfide bonds found in similar PA2 proteins.
  • Cellular localization
    Secreted.
  • Information by UniProt

Images

  • 12% SDS-PAGE separation of ab63846. Molecular Weight: ~15.5 kDa.

    Lane 1. Molecular weight marker
    Lane 2. reduced and heated sample, 5µg/lane
    Lane 3. non-reduced and non-heated sample, 5µg/lane

References

This product has been referenced in:
  • López-Sagaseta J  et al. sPLA2-V inhibits EPCR anticoagulant and antiapoptotic properties by accommodating lysophosphatidylcholine or PAF in the hydrophobic groove. Blood 119:2914-21 (2012). Human . Read more (PubMed: 22167755) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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