The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Additional notesProduct is NOT sterile! Please filter the product by an appropriate sterile filter before using it in cell culture.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
0.05M Acetate buffer pH 4.0
ReconstitutionAdd 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10µg/ml. In higher concentrations the solubility of this antigen is limited.
Ca2+ dependent phospholipase A2
Calcium dependent phospholipase A2
Calcium-dependent phospholipase A2
Group V phospholipase A2
Phosphatidylcholine 2 acylhydrolase
Phosphatidylcholine 2-acylhydrolase 5
Phospholipase A2 group V
sPLA2 Type V
FunctionPA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. This isozyme hydrolyzes more efficiently L-alpha-1-palmitoyl-2-oleoyl phosphatidylcholine than L-alpha-1-palmitoyl-2-arachidonyl phosphatidylcholine, L-alpha-1-palmitoyl-2-arachidonyl phosphatidylethanolamine, or L-alpha-1-stearoyl-2-arachidonyl phosphatidylinositol. May be involved in the production of lung surfactant, the remodeling or regulation of cardiac muscle.
Tissue specificityHeart, placenta and less abundantly, in lung.
Sequence similaritiesBelongs to the phospholipase A2 family.
Post-translational modificationsThis enzyme lacks one of the seven disulfide bonds found in similar PA2 proteins.
Recombinant Human Secretory phospholipase A2 Type V protein images
SDS-PAGE - Secretory phospholipase A2 Type V protein (His tag) (ab63846)
12% SDS-PAGE separation of ab63846. Molecular Weight: ~15.5 kDa.
Lane 1. Molecular weight marker
Lane 2. reduced and heated sample, 5µg/lane
Lane 3. non-reduced and non-heated sample, 5µg/lane
References for Recombinant Human Secretory phospholipase A2 Type V protein (ab63846)
This product has been referenced in:
López-Sagaseta J et al. sPLA2-V inhibits EPCR anticoagulant and antiapoptotic properties by accommodating lysophosphatidylcholine or PAF in the hydrophobic groove. Blood119:2914-21 (2012).
Read more (PubMed: 22167755) »