Recombinant Human Securin protein (ab87664)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      MGSSHHHHHH SSGLVPRGSH MATLIYVDKE NGEPGTRVVA KDGLKLGSGP SIKALDGRSQ VSTPRFGKTF DAPPALPKAT RKALGTVNRA TEKSVKTKGP LKQKQPSFSA KKMTEKTVKA KSSVPASDDA YPEIEKFFPF NPLDFESFDL PEEHQIAHLP LSGVPLMILD EERELEKLFQ LGPPSPVKMP SPPWESNLLQ SPSSILSTLD VELPPVCCDI DI

Specifications

Our Abpromise guarantee covers the use of ab87664 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Endotoxin level
    < 1.000 Eu/µg
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.316% Tris HCl, 20% Glycerol, 0.58% Sodium chloride, 0.0017% PMSF, 0.029% EDTA

General Info

  • Alternative names
    • AW555095
    • C87862
    • Cut2
    • EAP 1
    • EAP1
    • ESP1 associated protein 1
    • Esp1-associated protein
    • hPTTG
    • MGC126883
    • MGC138276
    • Pds1
    • Pituitary tumor transforming 1
    • Pituitary tumor transforming protein 1
    • Pituitary tumor-transforming 1, isoform CRA_a
    • Pituitary tumor-transforming 1, isoform CRA_b
    • Pituitary tumor-transforming gene 1
    • Pituitary tumor-transforming gene 1 protein
    • PTTG
    • PTTG 1
    • PTTG1
    • PTTG1 protein
    • PTTG1_HUMAN
    • Pttg3
    • Securin
    • Tumor transforming 1
    • Tumor transforming protein 1
    • Tumor-transforming protein 1
    • TUTR 1
    • TUTR1
    see all
  • Function
    Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation.
  • Tissue specificity
    Expressed at low level in most tissues, except in adult testis, where it is highly expressed. Overexpressed in many patients suffering from pituitary adenomas, primary epithelial neoplasias, and esophageal cancer.
  • Sequence similarities
    Belongs to the securin family.
  • Developmental stage
    Low level during G1 and S phases. Peaks at M phase. During anaphase, it is degraded.
  • Domain
    The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.
    The TEK-boxes are required for 'Lys-11'-linked ubiquitination and facilitate the transfer of the first ubiquitin and ubiquitin chain nucleation. TEK-boxes may direct a catalytically competent orientation of the UBE2C/UBCH10-ubiquitin thiolester with the acceptor lysine residue.
  • Post-translational
    modifications
    Phosphorylated at Ser-165 by CDK1 during mitosis.
    Phosphorylated in vitro by ds-DNA kinase.
    Ubiquitinated through 'Lys-11' linkage of ubiquitin moieties by the anaphase promoting complex (APC) at the onset of anaphase, conducting to its degradation. 'Lys-11'-linked ubiquitination is mediated by the E2 ligase UBE2C/UBCH10.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt

Images

  • ab87664 on 15% SDS-PAGE (3µg)

References

ab87664 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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