Recombinant human Semaphorin 4D/CD100 protein (ab168894)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human Semaphorin 4D/CD100 protein
See all Semaphorin 4D/CD100 proteins and peptides -
Biological activity
Measured by its ability to promote the survival and differentiation of Human peripheral blood monocytes. The ED50 for this effect is typically 2.0-6.3 µg/ml. -
Purity
> 95 % SDS-PAGE. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MAFAPIPRITWEHREVHLVQFHEPDIYNYSALLLSEDKDTLYIGAREAVF AVNALNISEKQHEVYWKVSEDKKAKCAEKGKSKQTECLNYIRVLQPLSAT SLYVCGTNAFQPACDHLNLTSFKFLGKNEDGKGRCPFDPAHSYTSVMVDG ELYSGTSYNFLGSEPIISRNSSHSPLRTEYAIPWLNEPSFVFADVIRKSP DSPDGEDDRVYFFFTEVSVEYEFVFRVLIPRIARVCKGDQGGLRTLQKKW TSFLKARLICSRPDSGLVFNVLRDVFVLRSPGLKVPVFYALFTPQLNNVG LSAVCAYNLSTAEEVFSHGKYMQSTTVEQSHTKWVRYNGPVPKPRPGACI DSEARAANYTSSLNLPDKTLQFVKDHPLMDDSVTPIDNRPRLIKKDVNYT QIVVDRTQALDGTVYDVMFVSTDRGALHKAISLEHAVHIIEETQLFQDFE PVQTLLLSSKKGNRFVYAGSNSGVVQAPLAFCGKHGTCEDCVLARDPYCA WSPPTATCVALHQTESPSRGLIQEMSGDASVCPDKSKGSYRQHFFKHGGT AELKCSQKSNLARVFWKFQNDVLKAESPKYGLMGRKNLLIFNLSEGDSGV YQCLSEERVKNKTVFQVVAKHVLEVKVVPKPVVAPTLSVVQTEGSRIATK VLVASTQGSSPPTPAVQATSSGAITLPPKPAPTGTSCEPKIVINTVPQLH SEKTMYLKSSDNR -
Predicted molecular weight
105 kDa -
Amino acids
22 to 734
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Specifications
Our Abpromise guarantee covers the use of ab168894 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Lyophilized -
Additional notes
This product was previously labelled as Semaphorin 4D
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C.
pH: 7.4
Constituents: 5% Trehalose, 0.75% Glycine, 0.61% TrisThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionIt is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 250 ug/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.
General Info
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Alternative names
- A8
- BB 18
- BB18
see all -
Function
May play a functional role in the immune system, as well as in the nervous system. Induces B-cells to aggregate and improves their viability in vitro. -
Tissue specificity
Strongly expressed in skeletal muscle, peripheral blood lymphocytes, spleen, and thymus and also expressed at lower levels in testes, brain, kidney, small intestine, prostate, heart, placenta, lung and pancreas, but not in colon and liver. -
Sequence similarities
Belongs to the semaphorin family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 PSI domain.
Contains 1 Sema domain. -
Cellular localization
Membrane. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab168894 has not yet been referenced specifically in any publications.