Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      LTE DMEKEISNAL GHGPQDEILS SAFKLRITRG DIQTLKNYHW LNDEVINFYM NLLVERNKKQ GYPALHVFST FFYPKLKSGG YQAVKRWTKG VNLFEQEIIL VPIHRKVHWS LVVIDLRKKC LKYLDSMGQK GHRICEILLQ YLQDESKTKR NSDLNLLEWT HHSMKPHEIP QQLNGSDCGM FTCKYADYIS RDKPITFTQH QMPLFRKKMV WEILHQQLL
    • Molecular weight
      29 kDa including tags
    • Amino acids
      368 to 589
    • Tags
      His tag N-Terminus
    • Additional sequence information
      Catalytic domain (NP_067640).

Specifications

Our Abpromise guarantee covers the use of ab188720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Typical enzyme concentration to support hydrolysis of substrates in vitro is 50- 500 nM depending on conditions. Pre-incubation (15 min) with 10 mM DTT is recommended to achieve maximum activity.

  • Applications

    SDS-PAGE

    Functional Studies

  • Purity
    >95% by SDS-PAGE .

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8
    Constituents: 1.19% HEPES, 0.58% Sodium chloride, 10% Glycerol, 0.08% DTT

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Axam2
    • DKFZp762A2316
    • KIAA1331
    • Senp2
    • SENP2_HUMAN
    • Sentrin specific protease 2
    • Sentrin-specific protease 2
    • Sentrin/SUMO specific protease SENP2
    • Sentrin/SUMO-specific protease SENP2
    • SMT3 specific isopeptidase 2
    • SMT3-specific isopeptidase 2
    • Smt3ip2
    • SUMO1/sentrin/SMT3 specific peptidase 2
    see all
  • Function
    Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO1, SUMO2 and SUMO3 to their mature forms and deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins. May down-regulate CTNNB1 levels and thereby modulate the Wnt pathway.
  • Sequence similarities
    Belongs to the peptidase C48 family.
  • Domain
    The N-terminus is necessary and sufficient for nuclear envelope targeting.
  • Post-translational
    modifications
    Polyubiquitinated; which leads to proteasomal degradation.
  • Cellular localization
    Nucleus > nuclear pore complex. Nucleus membrane. Cytoplasm. Shuttles between cytoplasm and nucleus.
  • Information by UniProt

References

ab188720 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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