Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MGSSHHHHHH SSGLVPRGSH MDPVVLSYMD SLLRQSDVSL LDPPSWLNDH IIGFAFEYFA NSQFHDCSDH VSFISPEVTQ FIKCTSNPAE IAMFLEPLDL PNKRVVFLAI NDNSNQAAGG THWSLLVYLQ DKNSFFHYDS HSRSNSVHAK QVAEKLEAFL GRKGDKLAFV EEKAPAQQNS YDCGMYVICN TEALCQNFFR QQTESLLQLL TPAYITKKRG EWKDLITTLA KK
    • Molecular weight
      26 kDa
    • Amino acids
      1 to 212
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab109837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF
  • Purity
    > 95 % SDS-PAGE.
    No detergents such as urea, Triton or Tween were used to purify the proteins. Purified by using anion exchange chromatography (DEAE sepharose resin) and gel filtration chromatography (Sephacryl S 200) with 20mM Tris pH 7.5, 2mM EDTA.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.32% Tris HCl, 0.58% Sodium chloride, 10% Glycerol, 0.02% DTT

General Info

  • Alternative names
    • cysteine 2
    • DEN 1
    • DEN1
    • Deneddylase 1
    • Deneddylase-1
    • FKSG8
    • HsT17512
    • NEDD 8 specific protease 1
    • NEDD8 specific protease cysteine 2
    • NEDD8-specific protease 1
    • NEDP 1
    • NEDP1
    • Protease
    • Protease, cysteine 2
    • PRSC2
    • SENP8
    • SENP8_HUMAN
    • Sentrin / SUMO specific protease SENP 8
    • Sentrin-specific protease 8
    • Sentrin/SUMO-specific protease SENP8
    • SUMO sentrin specific protease family member 8
    • SUMO/sentrin peptidase family member, NEDD8 specific
    • SUMO/sentrin specific peptidase family member 8
    see all
  • Function
    Protease that catalyzes two essential functions in the NEDD8 pathway: processing of full-length NEDD8 to its mature form and deconjugation of NEDD8 from targeted proteins such as cullins or p53.
  • Tissue specificity
    Broadly expressed, with highest levels in kidney and pancreas.
  • Sequence similarities
    Belongs to the peptidase C48 family.
  • Information by UniProt

Images

  • SDS-PAGE analysis of ab109837: (1) MW marker, (2) ab109837 at 3µg
    Gel concentration: 15%

References

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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