Recombinant human Serine/threonine-protein kinase 4 (ab60337)

Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • SpeciesHuman

Specifications

Our Abpromise guarantee covers the use of ab60337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • FormLiquid
  • Additional notes

    ab204853 (IRS1 peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Kinase responsive to stress
    • Krs2
    • Mammalian STE20 like protein kinase 1
    • Mammalian STE20-like protein kinase 1
    • Mammalian sterile 20 like 1
    • MST-1
    • MST1
    • Serine/threonine kinase 4
    • Serine/threonine protein kinase Krs 2
    • Serine/threonine-protein kinase 4
    • Serine/threonine-protein kinase Krs-2
    • STE20 like kinase MST1
    • STE20-like kinase MST1
    • STK4
    • STK4_HUMAN
    • TIIAC
    • YSK3
    see all
  • FunctionStress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. MST1/MST2 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation (By similarity). Phosphorylates 'Ser-14' of histone H2B (H2BS14ph) during apoptosis. Phosphorylates FOXO3 upon oxidative stress, which results in its nuclear translocation and cell death initiation.
  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SARAH domain.
  • Post-translational
    modifications
    Autophosphorylated on serine and threonine residues.
  • Cellular localizationCytoplasm. Nucleus. The caspase-cleaved form cycles between the nucleus and cytoplasm.
  • Information by UniProt

Recombinant human Serine/threonine-protein kinase 4 images

  • Sample Kinase Activity Plot.
  • ab60337 on SDS-PAGE, MW ~83kDa.

References for Recombinant human Serine/threonine-protein kinase 4 (ab60337)

ab60337 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry.

I can confirm that ab60337 MST1 protein (Active) has been phosphorylated. However, we have not done any in-house characterization studies to assess the state of activation/phosphorylation on specific sites. We hav...

Read More

Thank you for your inquiry.
Please find the attached for the full sequence information for ab60337.
As far as the kinase is concerned, we do know of various sites are phosphorylated. However, we have not done any in-house characterization stu...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"