Recombinant Human SETMAR protein (ab125543)

Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      69 kDa including tags
    • Amino acids
      1 to 671

Associated products

Specifications

Our Abpromise guarantee covers the use of ab125543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity
    > 85 % SDS-PAGE.
    Purity was determined to be >85% by densitometry.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

General Info

  • Alternative names
    • Histone lysine N methyltransferase
    • Histone lysine N methyltransferase SETMAR
    • Hsmar 1
    • Hsmar1
    • Mariner transposase Hsmar1
    • Metnase
    • SET domain and mariner transposase fusion
    • SET domain and mariner transposase fusion gene
    • SET domain and mariner transposase fusion gene containing protein
    • SET domain and mariner transposase fusion gene-containing protein
    • Setmar
    • SETMR_HUMAN
    see all
  • Function
    Histone methyltransferase that methylates 'Lys-4' and 'Lys-36' of histone H3, 2 specific tags for epigenetic transcriptional activation. Specifically mediates dimethylation of H3 'Lys-36'. Has sequence-specific DNA-binding activity and recognizes the 19-mer core of the 5'-terminal inverted repeats (TIRs) of the Hsmar1 element. Has DNA nicking activity. Has in vivo end joining activity and may mediate genomic integration of foreign DNA.
  • Tissue specificity
    Widely expressed, with highest expression in placenta and ovary and lowest expression in skeletal muscle.
  • Sequence similarities
    In the N-terminal section; belongs to the histone-lysine methyltransferase family.
    In the C-terminal section; belongs to the mariner transposase family.
    Contains 1 post-SET domain.
    Contains 1 pre-SET domain.
    Contains 1 SET domain.
  • Domain
    The mariner transposase Hsmar1 region mediates DNA-binding. It has no transposase activity because the active site contains an Asn in position 610 instead of a Asp residue.
  • Cellular localization
    Nucleus. Chromosome.
  • Information by UniProt

Images

  • SDS-PAGE analysis of ab125543.

References

ab125543 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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