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Product name Recombinant Human SF3B14 protein
Protein length Full length protein Description
Source Escherichia coli
Amino Acid Sequence
Sequence MGSSHHHHHH SSGLVPRGSH MAMQAAKRAN IRLPPEVNRI LYIRNLPYKI TAEEMYDIFG KYGPIRQIRV GNTPETRGTA YVVYEDIFDA
KNACDHLSGF NVCNRYLVVL YYNANRAFQK MDTKKKEEQL KLLKEKYGIN TDPPK
Molecular weight 17 kDa including tags
Amino acids 1 to 125
Tags His tag N-Terminus Specifications
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Purity > 95
% SDS-PAGE. Purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.
Concentration information loading... Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 30% Glycerol, 5mM DTT, 200mM Sodium chloride, 20mM Tris HCl, 1mM EDTA, pH 8
Function Necessary for the splicing of pre-mRNA. Directly contacts the pre-mRNA branch site adenosine for the first catalytic step of splicing. Enters the spliceosome and associates with the pre-mRNA branch site as part of the 17S U2 or, in the case of the minor spliceosome, as part of the 18S U11/U12 snRNP complex, and thus may facilitate the interaction of these snRNP with the branch sites of U2 and U12 respectively.
Sequence similarities Contains 1 RRM (RNA recognition motif) domain.
Cellular localization Nucleus.
Information by UniProt
Recombinant Human SF3B14 protein images
References for Recombinant Human SF3B14 protein (ab103908)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"