Recombinant Human SF3B14 protein (ab103908)

Overview

  • Product nameRecombinant Human SF3B14 protein
  • Protein lengthFull length protein

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionQ9Y3B4
    • SpeciesHuman
    • SequenceMGSSHHHHHH SSGLVPRGSH MAMQAAKRAN IRLPPEVNRI LYIRNLPYKI TAEEMYDIFG KYGPIRQIRV GNTPETRGTA YVVYEDIFDA KNACDHLSGF NVCNRYLVVL YYNANRAFQK MDTKKKEEQL KLLKEKYGIN TDPPK
    • Molecular weight17 kDa including tags
    • Amino acids1 to 125
    • TagsHis tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab103908 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Mass Spectrometry

    SDS-PAGE

  • Mass spectrometry
    MALDI-TOF
  • Purity> 95 % SDS-PAGE.
    Purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 30% Glycerol, 5mM DTT, 200mM Sodium chloride, 20mM Tris HCl, 1mM EDTA, pH 8

General Info

  • Alternative names
    • Ht006
    • P14
    • PM14_HUMAN
    • Pre mRNA branch site protein p14
    • Pre-mRNA branch site protein p14
    • SAP14
    • SF3B 14 kDa subunit
    • SF3B14
    • SF3B14a
    • Splicing factor 3B, 14 kDa subunit
    see all
  • FunctionNecessary for the splicing of pre-mRNA. Directly contacts the pre-mRNA branch site adenosine for the first catalytic step of splicing. Enters the spliceosome and associates with the pre-mRNA branch site as part of the 17S U2 or, in the case of the minor spliceosome, as part of the 18S U11/U12 snRNP complex, and thus may facilitate the interaction of these snRNP with the branch sites of U2 and U12 respectively.
  • Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
  • Cellular localizationNucleus.
  • Information by UniProt

Recombinant Human SF3B14 protein images

  • 15% SDS-PAGE analysis of ab103908 (3 µg)

References for Recombinant Human SF3B14 protein (ab103908)

ab103908 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab103908.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"