Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceM-GFWEEFES LQKQEVKNLH QRLEGQRPEN KGKNRYKNIL PFDHSRVILQ GRDSNIPGSD YINANYIKNQ LLGPDENAKT YIASQGCLEA TVNDFWQMAW QENSRVIVMT TREVEKGRNK CVPYWPEVGM QRAYGPYSVT NCGEHDTTEY KLRTLQVSPL DNGDLIREIW HYQYLSWPDH GVPSEPGGVL SFLDQINQRQ ESLPHAGPII VHCSAGIGRT GTIIVIDMLM ENISTKGLDC DIDIQKTIQM VRAQRSGMVQ TEAQYKFIYV AIAQFIETTK KKLEVLQSQK GQESEYGNIT Y

Specifications

Our Abpromise guarantee covers the use of ab51289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activitySpecific activity: > 5,000 units/mg of SHP-1. [Unit Definition : One unit will hydrolyze 1 nanomole of p-nitrophenylphosphatate per minute at pH 7.5 at 37°C using 10mM of substrate.]
  • Applications

    Phosphatase Activity

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.
    ab51289 was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer.
  • FormLiquid
  • Additional notes


    The catalytic domain of SHP-1 was overexpressed as insoluble protein aggregates (inclusion bodies).

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 20% Glycerol, 25mM Tris HCl, 2mM Beta mercaptoethanol, 1mM EDTA, 1mM DTT, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • 70Z-SHP
    • EC 3.1.3.48
    • HCP
    • HCPH
    • Hematopoietic cell phosphatase
    • Hematopoietic cell protein tyrosine phosphatase
    • Hematopoietic cell protein-tyrosine phosphatase
    • HPTP1C
    • Protein tyrosine phosphatase 1C
    • Protein tyrosine phosphatase non receptor type 6
    • Protein tyrosine phosphatase SHP1
    • Protein-tyrosine phosphatase 1C
    • protein-tyrosine phosphatase SHP 1
    • Protein-tyrosine phosphatase SHP-1
    • PTN6_HUMAN
    • PTP 1C
    • PTP-1C
    • PTP1C
    • Ptpn6
    • SH PTP 1
    • SH PTP1
    • SH-PTP1
    • SHP 1
    • SHP 1L
    • SHP1
    • SHP1L
    • tyrosine protein phosphatase non receptor type 6
    • Tyrosine-protein phosphatase non-receptor type 6
    see all
  • FunctionPlays a key role in hematopoiesis. This PTPase activity may directly link growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation.
  • Tissue specificityIsoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.
  • Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
    Contains 2 SH2 domains.
    Contains 1 tyrosine-protein phosphatase domain.
  • Post-translational
    modifications
    Phosphorylated on serine and tyrosine residues.
  • Cellular localizationCytoplasm. Nucleus. In neurons, translocates into the nucleus after treatment with angiotensin II.
  • Information by UniProt

Recombinant human SHP1 protein images

References for Recombinant human SHP1 protein (ab51289)

ab51289 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"