The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activitySpecific activity: > 5,000 units/mg of SHP-1. [Unit Definition : One unit will hydrolyze 1 nanomole of p-nitrophenylphosphatate per minute at pH 7.5 at 37°C using 10mM of substrate.]
% SDS-PAGE. ab51289 was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer.
The catalytic domain of SHP-1 was overexpressed as insoluble protein aggregates (inclusion bodies).
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Hematopoietic cell phosphatase
Hematopoietic cell protein tyrosine phosphatase
Hematopoietic cell protein-tyrosine phosphatase
Protein tyrosine phosphatase 1C
Protein tyrosine phosphatase non receptor type 6
Protein tyrosine phosphatase SHP1
Protein-tyrosine phosphatase 1C
protein-tyrosine phosphatase SHP 1
Protein-tyrosine phosphatase SHP-1
SH PTP 1
tyrosine protein phosphatase non receptor type 6
Tyrosine-protein phosphatase non-receptor type 6
FunctionPlays a key role in hematopoiesis. This PTPase activity may directly link growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation.
Tissue specificityIsoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily. Contains 2 SH2 domains. Contains 1 tyrosine-protein phosphatase domain.
Post-translational modificationsPhosphorylated on serine and tyrosine residues.
Cellular localizationCytoplasm. Nucleus. In neurons, translocates into the nucleus after treatment with angiotensin II.