Our Abpromise guarantee covers the use of ab54333 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Specific Activity: 6 U/ug. One Unit =1 pmol/min, Assay condition: 25 mM Tris/Cl, pH8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 30 uM BPS HDAC substrate , 200 uM NAD+, and 20 ng/ul Sirtuin 3. Incubation condition: 30 min at 30ºC.
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 10% Glycerol, 50mM Tris HCl, 20mM Glutathione, 138mM Sodium chloride, pH 8.0
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ab54333 has not yet been referenced specifically in any publications.