The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
>95% by SDS-PAGE .
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
NAD dependent deacetylase sirtuin 4
NAD dependent protein deacetylase sirtuin 4
NAD-dependent ADP-ribosyltransferase sirtuin-4
NAD-dependent protein deacetylase sirtuin-4
NAD-dependent protein lipoamidase sirtuin-4, mitochondrial
Regulatory protein SIR2 homolog 4
Silent mating type information regulation 2 homolog
Sir 2 like 4
SIR 2 like protein 4
Sir2 like 4
SIR2 like protein 4
SIR2-like protein 4
Sirtuin (silent mating type information regulation 2 homolog) 4
Sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae)
Sirtuin type 4
Acts as NAD-dependent protein lipoamidase, ADP-ribosyl transferase and deacetylase. Catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications. Inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner (PubMed:25525879). Catalyzes the transfer of ADP-ribosyl groups onto target proteins, including mitochondrial GLUD1, inhibiting GLUD1 enzyme activity. Acts as a negative regulator of mitochondrial glutamine metabolism by mediating mono ADP-ribosylation of GLUD1: expressed in response to DNA damage and negatively regulates anaplerosis by inhibiting GLUD1, leading to block metabolism of glutamine into tricarboxylic acid cycle and promoting cell cycle arrest (PubMed:16959573, PubMed:17715127). In response to mTORC1 signal, SIRT4 expression is repressed, promoting anaplerosis and cell proliferation. Acts as a tumor suppressor (PubMed:23562301, PubMed:23663782). Also acts as a NAD-dependent protein deacetylase: mediates deacetylation of 'Lys-471' of MLYCD, inhibiting its activity, thereby acting as a regulator of lipid homeostasis (By similarity). Controls fatty acid oxidation by inhibiting PPARA transcriptional activation. Impairs SIRT1:PPARA interaction probably through the regulation of NAD(+) levels (PubMed:24043310). Down-regulates insulin secretion.
Detected in vascular smooth muscle and striated muscle. Detected in insulin-producing beta-cells in pancreas islets of Langerhans (at protein level). Widely expressed. Weakly expressed in leukocytes and fetal thymus.
Belongs to the sirtuin family. Class II subfamily. Contains 1 deacetylase sirtuin-type domain.