Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      39 kDa including tags
    • Amino acids
      1 to 370
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab101134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    The specific activity of ab101134 was determined to be 20 RLU/min/ng.
  • Applications

    SDS-PAGE

    Functional Studies

  • Purity
    > 80 % SDS-PAGE.
    The purity was determined to be >80% by densitometry
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: 150mM Imidazole
    Constituents: 25% Glycerol, 50mM Sodium phosphate, 300mM Sodium chloride, 0.25mM DTT, 0.1mM PMSF, pH 7

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • NAD dependent deacetylase sirtuin 5
    • NAD dependent lysine demalonylase and desuccinylase sirtuin 5 mitochondrial
    • NAD dependent protein deacylase sirtuin 5 mitochondrial
    • NAD-dependent protein deacylase sirtuin-5, mitochondrial
    • Regulatory protein SIR2 homolog 5
    • Silent mating type information regulation 2 S.cerevisiae homolog 5
    • Sir2 like 5
    • SIR2-like protein 5
    • SIR2L5
    • SIR5_HUMAN
    • Sirt5
    • Sirtuin 5
    • Sirtuin type 5
    see all
  • Function
    NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins (PubMed:21908771, PubMed:22076378, PubMed:24703693). Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting (PubMed:22076378, PubMed:24703693). Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species (PubMed:24140062). Modulates ketogenesis through the desuccinylation and activation of HMGCS2 (By similarity). Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as UOX.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Belongs to the sirtuin family. Class III subfamily.
    Contains 1 deacetylase sirtuin-type domain.
  • Domain
    In contrast to class I sirtuins, class III sirtuins have only weak deacetylase activity. Difference in substrate specificity is probably due to a larger hydrophobic pocket with 2 residues (Tyr-102 and Arg-105) that bind to malonylated and succinylated substrates and define the specificity (PubMed:22076378).
  • Cellular localization
    Mitochondrion; Cytoplasm. Mitochondrion and Mitochondrion matrix. Mitochondrion intermembrane space. Cytoplasm, cytosol. Nucleus. Mainly mitochondrial. Also present extramitochondrially: a fraction is present in the cytosol and very small amounts are also detected in the nucleus.
  • Information by UniProt

Images

  • SDS-PAGE image showing ab101134 at ~39 kDa.
  • The specific activity of ab101134 was determined to be 20 RLU/min/ng.

References

ab101134 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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