Overview

Description

  • NatureRecombinant
  • SourceWheat germ
  • Amino Acid Sequence
    • AccessionQ13309
    • SpeciesHuman
    • SequenceEHFSPFRVQHMDLSNSVIEVSTLHGILSQCSKLQNLSLEGLRLSDPIVNT LAKNSNLVRLNLSGCSGFSEFALQTLLSSCSRLDELNLSWCFDFTEKHVQ VAVAHVSETI
    • Molecular weight38 kDa including tags
    • Amino acids176 to 285

Specifications

Our Abpromise guarantee covers the use of ab114386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    ELISA

    Western blot

  • FormLiquid
  • Additional notesProtein concentration is above or equal to 0.05 mg/ml.
    ab114386 is best used within three months from the date of receipt.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.79% Tris HCl, 0.3% Glutathione

General Info

  • Alternative names
    • CDK2/Cyclin A associated protein p45
    • Cyclin A/CDK2 associated protein p45
    • Cyclin-A/CDK2-associated protein p45
    • F box protein Skp2
    • F box/LRR repeat protein 1
    • F-box protein Skp2
    • F-box/LRR-repeat protein 1
    • FBL 1
    • FBL1
    • FBXL 1
    • FBXL1
    • FLB 1
    • FLB1
    • MGC1366
    • p45
    • p45skp2
    • S phase kinase associated protein 2
    • S phase kinase associated protein 2 (p45)
    • S-phase kinase-associated protein 2
    • S-phase kinase-associated protein 2 E3 ubiquitin protein ligase
    • SKP 2
    • Skp2
    • SKP2_HUMAN
    see all
  • FunctionSubstrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, MLL, CDK9, RAG2, FOXO1A, UBP43, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 F-box domain.
    Contains 9 LRR (leucine-rich) repeats.
  • Information by UniProt

Recombinant Human SKP2 protein images

  • ab114386 analysed on a 12.5% SDS-PAGE Stained with Coomassie Blue.

References for Recombinant Human SKP2 protein (ab114386)

ab114386 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"