Recombinant Human SLC4A4 protein (ab119144)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionQ9Y6R1
    • SpeciesHuman
    • SequenceMRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSTE NVEGKPSNLG ERGRARSSTF LRVVQPMFNH SIFTSAVSPA AERIRFILGEEDDSPAPPQL FTELDELLAV DGQEMEWKET ARWIKFEEKV EQGGERWSKP HVATLSLHSL FELRTCMEKG SIMLDREASS LPQLVEMIVDHQIETGLLKP ELKDKVTYTL LRKHRHQTKK SNLRSLADIG KTVSSASRMF TNPDNGSPAM THRNLTSSSL NDISDKPEKD QLKNKFMKKLPRDAEASNVL VGEVDFLDTP FIAFVRLQQA VMLGALTEVP VPTRFLFILL GPKGKAKSYH EIGRAIATLM SDEVFHDIAY KAKDRHDLIAGIDEFLDEVI VLPPGEWDPA IRIEPPKSLP SSDKRKNMYS GGENVQMNGD TPHDGGHGGG GHGDCEELQR TGRFCGGLIK DIKRKAPFFASDFYDALNIQ
    • Molecular weight52 kDa including tags
    • Amino acids1 to 424
    • TagsHis tag N-Terminus
    • Additional sequence informationSLC4A4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 460 amino acids (1-424 a.a.) and having a molecular mass of 51.5 kDa.

Specifications

Our Abpromise guarantee covers the use of ab119144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity> 90 % SDS-PAGE.
    ab119144 was purified by proprietary chromatographic techniques.
  • FormLiquid
  • Additional notesFor long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    pH: 7.50
    Constituents: 0.32% Tris HCl, 0.01% DTT, 0.002% PMSF, 10% Glycerol

General Info

  • Alternative names
    • DKFZp781H1314
    • Electrogenic sodium bicarbonate cotransporter 1
    • hhNMC
    • HNBC 1
    • HNBC1
    • KNBC
    • kNBC 1
    • kNBC1
    • Na(+)/HCO3(-) cotransporter
    • Na+HCO3- cotransporter 4
    • NBC 1
    • NBC 2
    • NBC1
    • NBC2
    • Nbc4
    • NBCE 1
    • NBCE1
    • OTTHUMP00000160355
    • OTTHUMP00000218884
    • OTTHUMP00000218885
    • PNBC
    • S4A4_HUMAN
    • SLC4A4
    • SLC4A5
    • Sodium bicarbonate cotransporter
    • Sodium bicarbonate cotransporter 1 (sodium bicarbonate cotransporter, kidney; sodium bicarbonate cotransporter, pancreas)
    • Sodium bicarbonate cotransporter kidney
    • sodium bicarbonate cotransporter member 4
    • Sodium bicarbonate cotransporter pancreas
    • Solute carrier family 4 member 4
    • Solute carrier family 4 sodium bicarbonate cotransporter member 4
    • Solute carrier family 4 sodium bicarbonate cotransporter member 5
    • Solute carrier family 4, sodium bicarbonate cotransporter, member 4, brain type
    see all
  • FunctionElectrogenic sodium/bicarbonate cotransporter with a Na(+):HCO3(-) stoichiometry varying from 1:2 to 1:3. May regulate bicarbonate influx/efflux at the basolateral membrane of cells and regulate intracellular pH.
  • Tissue specificityIsoform 1 is expressed in pancreas and to a lower extent in heart, skeletal muscle, liver, parotid salivary glands, prostate, colon, stomach, thyroid, brain and spinal chord. Corneal endothelium cells express only isoform 1 (at protein level). Isoform 2 is specifically expressed in kidney at the level of proximal tubules.
  • Involvement in diseaseDefects in SLC4A4 are the cause of proximal renal tubular acidosis with ocular abnormalities (pRTA-OA) [MIM:604278]; also known as renal tubular acidosis II. Caused by an impairment of bicarbonate absorption in the proximal tubule, proximal renal tubular acidosis (pRTA) is characterized by a decreased renal HCO3(-) threshold. pRTA-OA is an extremely rare autosomal recessive syndrome characterized by short stature, profound pRTA, mental retardation, bilateral glaucoma, cataracts and bandkeratopathy.
    Note=Loss of interaction with and stimulation by CA4 is the cause of retinitis pigmentosa type 17 (RP17).
  • Sequence similaritiesBelongs to the anion exchanger (TC 2.A.31) family.
  • Post-translational
    modifications
    Phosphorylation of Ser-1026 by PKA increases the binding of CA2 and changes the Na(+):HCO3(-) stoichiometry of the transporter from 3:1 to 2:1. Phosphorylation of Thr-49 regulates isoform 1 conductance.
    N-glycosylation is not necessary for the transporter basic functions.
  • Cellular localizationBasolateral cell membrane.
  • Information by UniProt

References for Recombinant Human SLC4A4 protein (ab119144)

ab119144 has not yet been referenced specifically in any publications.

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