Recombinant human SMURF 2 protein (ab95930)

Overview

  • Product nameRecombinant human SMURF 2 protein
  • Protein lengthProtein fragment

Description

  • NatureRecombinant
  • SourceBaculovirus
  • Amino Acid Sequence
    • AccessionQ9HAU4
    • SpeciesHuman
    • Molecular weight72 kDa including tags
    • Amino acids130 to 748
    • TagsDDDDK tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab95930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activitySpecific Activity: 0.00049 pmol/min/µg Assay conditions: 50 µl reaction mix (4 mM ATP, 5mM MgCl2, 1mM DTT, E1 (0.5 µg), E2 (2.8 µg), Biotin-Ubiquitin (0.5 µg) and SMURF 2 (0-2 µg) in 50mM Tris pH 7.4) incubate for 30min at 37°C, then add to streptavidin coated plates and incubate for 30min at 37°C. Add antibody against SMURF 2, incubate 1h. Finally, incubate 1h with the Europeum-labeled secondary antibody. Time resolved fluorescence measured at room temperature (Lambda exc=330 nm, Lambda em=620 nm).
  • Applications

    SDS-PAGE

    Functional Studies

  • Purity 80 % .
    Purity is >80% by SDS-PAGE.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Store at -80°C.

    pH: 8.00
    Constituents: 20% Glycerol, 0.58% Sodium chloride, 0.395% Tris HCl, 0.05% Tween, 0.0462% DTT
    Note: 102 µg/ml FLAG peptide

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • E3 ubiquitin-protein ligase SMURF2
    • EC 6.3.2.
    • hSMURF2
    • MGC138150
    • Smad specific E3 ubiquitin ligase 2
    • SMAD specific E3 ubiquitin protein ligase 2
    • SMAD ubiquitination regulatory factor 2
    • SMAD-specific E3 ubiquitin-protein ligase 2
    • SMUF2_HUMAN
    • Smurf2
    • Ubiquitin protein ligase SMURF2
    see all
  • FunctionE3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.
  • Tissue specificityWidely expressed.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 C2 domain.
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 3 WW domains.
  • DomainThe second and third WW domains are responsible for interaction with the PY-motif of R-SMAD (SMAD1, SMAD2 and SMAD3).
    The C2 domain is involved in autoinhibition of the catalytic activity by interacting with the HECT domain.
  • Post-translational
    modifications
    Auto-ubiquitinated and ubiquitinated in the presence of RNF11 and UBE2D1.
  • Cellular localizationNucleus. Cytoplasm. Cell membrane. Membrane raft. Cytoplasmic in the presence of SMAD7. Co-localizes with CAV1, SMAD7 and TGF-beta receptor in membrane rafts.
  • Information by UniProt

Recombinant human SMURF 2 protein images

  • Image showing specific activity of ab95930.
  • 10% SDS-PAGE
    Lane 1: ab95930 (4µg)
    Lane 2: Molecular weight markers

References for Recombinant human SMURF 2 protein (ab95930)

ab95930 has not yet been referenced specifically in any publications.

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