Recombinant human SMURF1 protein (ab95928)

Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      70 kDa including tags
    • Amino acids
      150 to 757
    • Tags
      DDDDK tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab95928 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Specific Activity: 0.0055 pmol/min/µg Assay conditions: 50 µl reaction mix (4mM ATP, 5mM MgCl2, 1mM DTT, E1 (0.5 µg), E2 (2.8 µg), Biotin-Ubiquitin (0.5 µg) and SMURF1 (0-1 µg) in 50mM Tris pH 7.4) incubate for 30min at 37°C, then add to streptavidin coated plates and incubate for 30min at 37°C. Add antibody against SMURF1, incubate 1h. Finally, incubate 1h with the Europeum-labeled secondary antibody. Time resolved fluorescence measured at room temperature (Lambda exc=330 nm, Lambda em=620 nm).
  • Applications

    SDS-PAGE

    Functional Studies

  • Purity
    80 % .
    Purity is >80% by SDS-PAGE.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Store at -80°C.

    pH: 8.00
    Constituents: 0.05% Tween, 0.0462% DTT, 20% Glycerol, 0.58% Sodium chloride, 0.395% Tris HCl
    Note: 101 µg/ml FLAG peptide

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • E3 ubiquitin-protein ligase SMURF1
    • hSMURF1
    • KIAA1625
    • Smad specific E3 ubiquitin ligase 1
    • SMAD specific E3 ubiquitin protein ligase 1
    • Smad ubiquitination regulatory factor 1
    • SMAD-specific E3 ubiquitin-protein ligase 1
    • SMUF1_HUMAN
    • SMURF 1
    • smurf1
    see all
  • Function
    E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Acts by mediating ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Contains 1 C2 domain.
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 2 WW domains.
  • Post-translational
    modifications
    Ubiquitinated by the SCF(FBXL15) complex at Lys-381 and Lys-383, leading to its degradation by the proteasome. Lys-383 is the primary ubiquitination site.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt

Images

  • Image showing specific activity of ab95928.
  • 10% SDS-PAGE
    Lane 1: ab95928 (3µg)
    Lane 2: Molecular weight markers

References

ab95928 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting us. The laboratory sent the following information:


Flag-SMURF1(150-) : pI=5.77



An acidic protein

I hope this information is helpful to you. Please do not hesitate to contact us if you n...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up