Recombinant human SMURF1 protein (ab95928)

Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      70 kDa including tags
    • Amino acids
      150 to 757
    • Tags
      DDDDK tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab95928 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Specific Activity: 0.0055 pmol/min/µg Assay conditions: 50 µl reaction mix (4mM ATP, 5mM MgCl2, 1mM DTT, E1 (0.5 µg), E2 (2.8 µg), Biotin-Ubiquitin (0.5 µg) and SMURF1 (0-1 µg) in 50mM Tris pH 7.4) incubate for 30min at 37°C, then add to streptavidin coated plates and incubate for 30min at 37°C. Add antibody against SMURF1, incubate 1h. Finally, incubate 1h with the Europeum-labeled secondary antibody. Time resolved fluorescence measured at room temperature (Lambda exc=330 nm, Lambda em=620 nm).
  • Applications

    SDS-PAGE

    Functional Studies

  • Purity
    80 % .
    Purity is >80% by SDS-PAGE.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Store at -80°C.

    pH: 8.00
    Constituents: 0.05% Tween, 0.0462% DTT, 20% Glycerol, 0.58% Sodium chloride, 0.395% Tris HCl
    Note: 101 µg/ml FLAG peptide

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • E3 ubiquitin-protein ligase SMURF1
    • hSMURF1
    • KIAA1625
    • Smad specific E3 ubiquitin ligase 1
    • SMAD specific E3 ubiquitin protein ligase 1
    • Smad ubiquitination regulatory factor 1
    • SMAD-specific E3 ubiquitin-protein ligase 1
    • SMUF1_HUMAN
    • SMURF 1
    • smurf1
    see all
  • Function
    E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Acts by mediating ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Contains 1 C2 domain.
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 2 WW domains.
  • Post-translational
    modifications
    Ubiquitinated by the SCF(FBXL15) complex at Lys-381 and Lys-383, leading to its degradation by the proteasome. Lys-383 is the primary ubiquitination site.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt

Recombinant human SMURF1 protein images

  • Image showing specific activity of ab95928.
  • 10% SDS-PAGE
    Lane 1: ab95928 (3µg)
    Lane 2: Molecular weight markers

References for Recombinant human SMURF1 protein (ab95928)

ab95928 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. The laboratory sent the following information:


Flag-SMURF1(150-) : pI=5.77



An acidic protein

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