Recombinant Human SMYD3 protein (ab80377)

Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • SpeciesHuman
    • Amino acids35 to 428

Associated products

Specifications

Our Abpromise guarantee covers the use of ab80377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 30% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8

General Info

  • Alternative names
    • bA74P14.1
    • bA74P14.1 (novel protein)
    • FLJ21080
    • histone lysine N methyltransferase SMYD3
    • KMT3E
    • MGC104324
    • SET and MYND domain containing 3
    • SET and MYND domain containing protein 3
    • SET and MYND domain-containing protein 3
    • SMYD 3
    • Smyd3
    • SMYD3 protein
    • SMYD3_HUMAN
    • Zinc finger MYND domain containing 1
    • Zinc finger MYND domain containing protein 1
    • Zinc finger MYND domain-containing protein 1
    • Zinc finger protein subfamily 3A MYND domain containing 1
    • Zinc finger protein, subfamily 3A (MYND domain containing), 1
    • ZMYND 1
    • ZMYND1
    • ZNFN3A1
    see all
  • FunctionHistone methyltransferase. Specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences.
  • Tissue specificityExpressed in skeletal muscles and testis. Overexpressed in a majority of colorectal and hepatocellular carcinomas.
  • Sequence similaritiesBelongs to the histone-lysine methyltransferase family.
    Contains 1 MYND-type zinc finger.
    Contains 1 SET domain.
  • Cellular localizationCytoplasm. Nucleus. Mainly cytoplasmic when cells are arrested at G0/G1. Accumulates in the nucleus at S phase and G2/M.
  • Information by UniProt

Recombinant Human SMYD3 protein images

  • Lane 1: ab80377 on 10% SDS-PAGE, Coomassie staining, 4µg.
    Lane 2: Protein marker.

References for Recombinant Human SMYD3 protein (ab80377)

ab80377 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your response.

We currently do not know of an accurate method to accurately determine the specific activity of SMYD3 enzyme. We are still in contact with the lab and will let you know should this information become available. ...

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Thank you for your email.

Unfortunately we do not currently have any antibodies directed towards the methylated K5 of histone 4. We only have antibodies to the acetylated targetof thisresidue (rabbit polyclonals http://www.abcam.com/Histone-H...

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Thank you for contacting us.

Unfortunately I have been informed that the fluorescence polarisation assay described on the datasheet of SMYD3 protein (Active) (ab80377) is not an accurate assay to determine the activity of SMYD3. We will be up...

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Thank you contacting us with these further questions. I have spoken with the lab about a usefull concentration for the purified HMT enzyme. Based on tests, we conclude that the useful concentration range can be from 10 ng to 200 ng/well (30 ul). ...

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