Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceMAEDADMRNE LEEMQRRADQ LADESLESTR RMLQLVEESK DAGIRTLVML DEQGEQLERI EEGMDQINKD MKEAEKNLTD LGKFCGLCVC PCNKLKSSDA YKKAWGNNQD GVVASQPARV VDEREQMAIS GGFIRRVTND ARENEMDENL EQVSGIIGNL RHMALDMGNE IDTQNRQIDR IMEKADSNKT RIDEANQRAT KMLGSG

Specifications

Our Abpromise guarantee covers the use of ab74529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

  • Purity> 90 % SDS-PAGE.
    ab74529 was purified by using conventional chromatography techniques.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0154% DTT, 0.3025% Tris, 10% Glycerol

General Info

  • Alternative names
    • bA416N4.2
    • Bdr
    • CMS18
    • dJ1068F16.2
    • FLJ23079
    • HGNC:11132
    • MGC105414
    • MGC139754
    • Resistance to inhibitors of cholinesterase 4 homolog
    • RIC 4
    • RIC4
    • SEC 9
    • SEC9
    • SNAP
    • SNAP 25
    • SNAP-25
    • SNAP-25B
    • SNAP25
    • SNP 25
    • SNP25
    • SNP25_HUMAN
    • sp
    • SUP
    • Super protein
    • Synaptosomal associated 25 kDa protein
    • Synaptosomal associated protein
    • Synaptosomal associated protein 25
    • Synaptosomal associated protein 25kDa
    • Synaptosomal-associated 25 kDa protein
    • Synaptosomal-associated protein 25
    • Synaptosomal-associated protein, 25-KD
    see all
  • Functiont-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.
  • Tissue specificityNeurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.
  • Sequence similaritiesBelongs to the SNAP-25 family.
    Contains 2 t-SNARE coiled-coil homology domains.
  • Post-translational
    modifications
    Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association.
  • Cellular localizationCytoplasm > perinuclear region. Cell membrane. Cell junction > synapse > synaptosome. Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region.
  • Information by UniProt

Recombinant Human SNAP25 protein images

  • 15% SDS-PAGE showing ab74529 (3µg)
  • Anti-SNAP25 antibody (ab41455) at 1 µg/ml + Recombinant Human SNAP25 protein (ab74529) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

References for Recombinant Human SNAP25 protein (ab74529)

This product has been referenced in:
  • Stura EA  et al. Structural Framework for Covalent Inhibition of Clostridium botulinum Neurotoxin A by Targeting Cys165. J Biol Chem 287:33607-14 (2012). SDS-PAGE . Read more (PubMed: 22869371) »

See 1 Publication for this product

Product Wall

I have been in contact with our lab and products team and indeed there is a duplication here which we are currently trying to fix.

I can confirm that ab74529 and ab155885 are identical and ab87335 and ab51048 are also identical.

The ...

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The laboratory said they would remove the EDTA from ab74529 via dialysis.
Alternatively, we have now added the same SNAP25 protein with EDTA removed:
ab155885: http://www.abcam.com/snap25-protein-edta-free-ab155885.html

Thank you for contacting us.

I have been in touch with the lab. While they have not run this protein in Western blot, it has been checked in SDS-PAGE and no additional bands have been seen. Would you be willing to provide and image of the ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"