The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Specific activity is > 1,200 units/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and Xanthine oxidase at pH 7.8 at 25°C in a 1.5 ml reaction volume.
Prepare a 1.5 ml reaction mix into a suitable container and pre-chill on ice before use: The final concentrations are 50mM potassium phosphate, 0.1mM ethylendiaminetetraacetic acid, 0.01mM cytochrome C, 0.05mM xanthine, 0.005 units xanthine oxidase.
Equilibrate to 25°C and monitor at A550nm until the value is constant using a spectrophotometer.
Add 50 ul of recombinant SOD protein in various concentrations (0.5 ug, 1 ug) in assay buffer.
Mix by inversion and record the increase at A550nm for 5 minutes.
% SDS-PAGE. ab93946 was purified using conventional chromatography techniques.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 20% Glycerol, 20mM Tris HCl, pH 8.0
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Manganese containing superoxide dismutase
Manganese superoxide dismutase
Mangano superoxide dismutase
Mn superoxide dismutase
Superoxide dismutase [Mn] mitochondrial
Superoxide dismutase [Mn], mitochondrial
Superoxide dismutase 2 mitochondrial
Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
Involvement in disease
Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6) [MIM:612634]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
Belongs to the iron/manganese superoxide dismutase family.
Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity.