• NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionQ15465
    • SpeciesHuman
    • Molecular weight20 kDa
    • Amino acids25 to 197


Our Abpromise guarantee covers the use of ab123773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityHuman recombinant Sonic Hedgehog is biologically active as compared to a standard. The ED50, measured by the dose-dependent induction of alkaline phosphatase production by C3H/10T1/2 fibroblasts, is 750ng/ml.
  • Applications


    Functional Studies

  • Purity> 95 % SDS-PAGE.
    Purity is greater than 97% as determined by HPLC and SDS-PAGE. Endotoxin Level: <0.01 ng/µg.
  • FormLyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20ºC.

    pH: 7.50
    Constituent: 0.14% Sodium phosphate

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionWhen reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water to a concentration of 0.1-0.5 mg/ml, which can be further diluted into other aqueous solutions. Upon reconstitution the material should be aliquoted and frozen at -20 °C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

General Info

  • Alternative names
    • HHG 1
    • HHG-1
    • HHG1
    • HLP 3
    • HLP3
    • Holoprosencephaly 3
    • HPE 3
    • HPE3
    • MCOPCB5
    • shh
    • SMMC I
    • SMMCI
    • Sonic Hedgehog (Drosophila) homolog
    • Sonic hedgehog homolog
    • sonic hedgehog homolog (Drosophila)
    • Sonic hedgehog protein
    • Sonic hedgehog protein C-product
    • TPT
    • TPTPS
    see all
  • FunctionBinds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction.
  • Tissue specificityExpressed in fetal intestine, liver, lung, and kidney. Not expressed in adult tissues.
  • Involvement in diseaseDefects in SHH are the cause of microphthalmia isolated with coloboma type 5 (MCOPCB5) [MIM:611638]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataract and other abnormalities like cataract may also be present. Ocular colobomas are a set of malformations resulting from abnormal morphogenesis of the optic cup and stalk, and the fusion of the fetal fissure (optic fissure).
    Defects in SHH are the cause of holoprosencephaly type 3 (HPE3) [MIM:142945]. Holoprosencephaly (HPE) [MIM:236100] is the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability. The majority of HPE3 cases are apparently sporadic, although clear examples of autosomal dominant inheritance have been described. Interestingly, up to 30% of obligate carriers of HPE3 gene in autosomal dominant pedigrees are clinically unaffected.
    Defects in SHH are a cause of solitary median maxillary central incisor (SMMCI) [MIM:147250]. SMMCI is a rare dental anomaly characterized by the congenital absence of one maxillary central incisor.
    Defects in SHH are the cause of triphalangeal thumb-polysyndactyly syndrome (TPTPS) [MIM:174500]. TPTPS is an autosomal dominant syndrome characterized by a wide spectrum of pre- and post-axial abnormalities due to altered SHH expression pattern during limb development. TPTPS mutations have been mapped to the 7q36 locus in the LMBR1 gene which contains in its intron 5 a long-range cis-regulatory element of SHH expression.
  • Sequence similaritiesBelongs to the hedgehog family.
  • Post-translational
    The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity.
    Cholesterylation is required for N-product targeting to lipid rafts and multimerization.
    N-palmitoylation of Cys-24 by HHAT is required for N-product multimerization and full activity.
  • Cellular localizationCell membrane. The N-product either remains associated with lipid rafts at the cell surface, or forms freely diffusible active multimers with its hydrophobic lipid-modified N- and C-termini buried inside and Secreted > extracellular space. The C-terminal peptide diffuses from the cell.
  • Information by UniProt

References for Recombinant human Sonic Hedgehog protein (ab123773)

This product has been referenced in:
  • Huang M  et al. Embelin suppresses growth of human pancreatic cancer xenografts, and pancreatic cancer cells isolated from KrasG12D mice by inhibiting Akt and Sonic hedgehog pathways. PLoS One 9:e92161 (2014). Read more (PubMed: 24694877) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab123773.
Please use the links above to contact us or submit feedback about this product.