Recombinant Human SOX2 protein (ab95847)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      34 kDa

Associated products

Specifications

Our Abpromise guarantee covers the use of ab95847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Dot blot

    SDS-PAGE

    Indirect ELISA

  • Purity
    > 90 % SDS-PAGE.
    ab95847 was refolded to soluble form.
  • Form
    Liquid
  • Additional notes
    This product is for Research Use Only and not to be used for any commercial resale purpose, including, but not limited to, using the product as part of any developed assay technology such as ELISA, ELISPOT, or Multiplex assay that is sold to another end-user.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 500mM L-arginine, 300mM Guanidine-HCl, 50mM HEPES, 5mM Cysteamine, 5mM Cystamine, 5mM Calcium chloride, 3mM DTT, pH 7.5

General Info

  • Alternative names
    • ANOP3
    • cb236
    • Delta EF2a
    • lcc
    • MCOPS3
    • MGC148683
    • MGC2413
    • RGD1565646
    • Sex determining region Y box 2
    • SOX 2
    • Sox2
    • SOX2_HUMAN
    • SRY (sex determining region Y) box 2
    • SRY box containing gene 2
    • SRY related HMG box 2
    • SRY related HMG box gene 2
    • SRY-box 2
    • Transcription factor SOX 2
    • Transcription factor SOX-2
    • ysb
    see all
  • Function
    Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency.
  • Involvement in disease
    Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.
  • Sequence similarities
    Contains 1 HMG box DNA-binding domain.
  • Post-translational
    modifications
    Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.
  • Cellular localization
    Nucleus.
  • Information by UniProt

Images

  • SDS-PAGE analysis of 1.5µg ab95847.
  • anti-SOX2 monoclonal at 1 µg/ml + Recombinant Human SOX2 protein (ab95847) at 0.06 µg
  • Indirect ELISA detection of immobilized ab95847 with titrated amounts of SOX2 Monoclonal Antibody

References

ab95847 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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