Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • AccessionQ9NYA1
    • SpeciesHuman
    • Molecular weight71 kDa including tags
    • Amino acids1 to 384

Specifications

Our Abpromise guarantee covers the use of ab125662 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityThe specific activity of ab125662 was determined to be 450 - 515 nmol/min/mg.
  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Purity> 70 % Densitometry.
    Purity was determined to be >70% by densitometry.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • SK 1
    • SK1
    • Sphingosine kinase 1
    • SPHK
    • SPHK 1
    • Sphk1
    • SPHK1_HUMAN
    • SPK
    • SPK 1
    see all
  • FunctionCatalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra-and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol.
  • Tissue specificityWidely expressed with highest levels in adult liver, kidney, heart and skeletal muscle.
  • Sequence similaritiesContains 1 DAGKc domain.
  • Information by UniProt

Recombinant human SPHK1 protein images

  • SDS-PAGE analysis of ab125662.
  • Sample Kinase Assay showing the specific activity of ab125662 as 450 nmol/min/mg.

References for Recombinant human SPHK1 protein (ab125662)

ab125662 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"