Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceMGSSHHHHHH SSGLVPRGSH MEGGLGRAVC LLTGASRGFG RTLAPLLASL LSPGSVLVLS ARNDEALRQL EAELGAERSG LRVVRVPADL GAEAGLQQLL GALRELPRPK GLQRLLLINN AGSLGDVSKG FVDLSDSTQV NNYWALNLTS MLCLTSSVLK AFPDSPGLNR TVVNISSLCA LQPFKGWALY CAGKAARDML FQVLALEEPN VRVLNYAPGP LDTDMQQLAR ETSVDPDMRK GLQELKAKGK LVDCKVSAQK LLSLLEKDEF KSGAHVDFYD K

Specifications

Our Abpromise guarantee covers the use of ab86445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.
    ab86445 is purified using conventional chromatography techniques.
  • FormLiquid
  • Additional notesEndotoxin Level: < 1.0 EU per 1 µg of protein (determined by LAL method).
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 20mM Tris HCl, pH 8.0

General Info

  • Alternative names
    • OTTHUMP00000160199
    • SDR38C1
    • Sepiapterin reductase
    • Sepiapterin reductase (7,8 dihydrobiopterin:NADP+ oxidoreductase)
    • Short chain dehydrogenase/reductase family 38C, member 1
    • SPR
    • SPRE_HUMAN
    see all
  • FunctionCatalyzes the final one or two reductions in tetra-hydrobiopterin biosynthesis to form 5,6,7,8-tetrahydrobiopterin.
  • Involvement in diseaseDefects in SPR are the cause of dystonia DOPA-responsive due to sepiapterin reductase deficiency (DRDSPRD) [MIM:612716]. In the majority of cases, patients manifest progressive psychomotor retardation, dystonia and spasticity. Cognitive anomalies are also often present. The disease is due to severe dopamine and serotonin deficiencies in the central nervous system caused by a defect in BH4 synthesis. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures.
  • Sequence similaritiesBelongs to the sepiapterin reductase family.
  • Post-translational
    modifications
    In vitro phosphorylation of Ser-213 by CaMK2 does not change kinetic parameters.
  • Cellular localizationCytoplasm.
  • Information by UniProt

Recombinant Human SPR protein images

  • 15% SDS-PAGE showing ab86445 at approximately 30.2kDa (3µg).

References for Recombinant Human SPR protein (ab86445)

ab86445 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"