Recombinant Human SPRR1b protein (ab167925)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MKHHHHHHAS SQQQKQPCTP PPQLQQQQVK QPCQPPPQEP CIPKTKEPCH PKVPEPCHPK VPEPCQPKVP EPCHPKVPEP CPSIVTPAPA QQKTKQK
    • Molecular weight
      11 kDa including tags
    • Amino acids
      2 to 89
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab167925 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

    SDS-PAGE

    Mass Spectrometry

  • Endotoxin level
    < 1.000 Eu/µg
  • Mass spectrometry
    LC-MS/MS
  • Purity
    > 95 % Densitometry.
    ab167925 was purifed using Ni-NTA chromatography.
  • Form
    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -80°C.

    pH: 7.40
    Constituents: 99% Phosphate Buffer, 0.43% Sodium chloride

  • Reconstitution
    Add 200µl of deionized water to prepare a working stock solution of 0.5 mg/ml and let the lyophilized pellet dissolve completely. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage.

    Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

General Info

  • Alternative names
    • 14.9 kDa pancornulin
    • Cornifin
    • Cornifin B
    • Cornifin-B
    • CornifinB
    • GADD33
    • MGC61901
    • Small proline rich protein IB
    • Small proline-rich protein IB
    • SPR-IB
    • SPR1B_HUMAN
    • SPRIB
    • SPRR1
    • SPRR1B
    see all
  • Function
    Cross-linked envelope protein of keratinocytes. It is a keratinocyte protein that first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase. All that results in the formation of an insoluble envelope beneath the plasma membrane. Can function as both amine donor and acceptor in transglutaminase-mediated cross-linkage.
  • Tissue specificity
    Suprabasal layers of squamous-differentiated tissues such as epidermis, esophagus, tongue and trachea.
  • Sequence similarities
    Belongs to the cornifin (SPRR) family.
  • Developmental stage
    Expressed during differentiation of squamous cells.
  • Post-translational
    modifications
    The N-terminus is blocked.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt

Images

  • 14% SDS-PAGE analysis of ab167925

    Lane 1: reduced and boiled sample, 2.5µg/lane
    Lane 2: non-reduced and non-boiled sample, 2.5µg/lane

References

ab167925 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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