The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Binds AMSH protein with Kd = ~7 μM. In in vitro assays containing 200 nM AMSH, a ~10-fold increase in the rate of hydrolysis of K63-linked diubiquitin FRET substrate was achieved upon the addition of STAM1. Half-maximal increase occurred at 0.4 μM STAM1.
>90% by SDS-PAGE.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Signal transducing adapter molecule 1
signal transducing adaptor molecule (SH3 domain and ITAM motif) 1
Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes.